Results 121 to 130 of about 820,234 (313)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Content and innovative methods of feedback organization in “a teacher – students” dual configuration

open access: yesStudia Humanitatis, 2021
The study defines the content and pedagogical feasibility of using innovative methods of feedback organizing in “a teacher – students” dual configuration. This is facilitated by the systematic application of numerous innovative methods: “It’s in my palm”,
Kubanov Ruslan Anatoliyovych
doaj  

NCRM podcast: Digital Methods as Mainstream Methodologies

open access: yes, 2013
In an era in which social life is increasingly played out online, innovative digital research methods are providing new ways of asking questions and generating data. But with exciting new opportunities come a number of complex challenges. In this podcast
Morey, Yvette   +2 more
core  

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

A new matrix method to innovation

open access: yesProcedia Computer Science, 2022
Hangzhou Li   +3 more
openaire   +1 more source

A brief history of the National Centre for Research Methods [PDF]

open access: yes
The National Centre for Research Methods (NCRM) is celebrating its 20th anniversary in 2024. This video timeline provides an overview of some of the centre's key milestones over the past two decades.
Grover, Ed
core  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

open access: yesFEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa   +2 more
wiley   +1 more source

The Role Of Modern Educational Technology In The Process Of Teaching Chinese And The Development Of Communicative Competence

open access: yesAstra Salvensis, 2017
The relevance of this work is determined by introducing new standards of teaching foreign languages as well as the severization of requirements to the quality of Chinese language acquisition by the students of higher educational institutions. The goal of
Alfiya R. ALIKBEROVA
doaj  

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