Results 91 to 100 of about 727,631 (337)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Test of Host Sanction Hypothesis in Soybean Plants Co-inoculated with Nitrogen Fixing and Non-fixing Bradyrhizobium japonicum [PDF]

, 2015
Aims: We tested the proposed mechanism for potential sanctions, that the plant would reduce viability of non-fixing rhizobia inside nodules, performing viable Bradyrhizobium japonicum counts from co-occupied and single-occupied nodules in co-inoculated ...
Bedmar, Eulogio, Marco, Diana Elizabeth, Talbi, Chouhra   +2 more
core   +1 more source

Day/night variations of myeloid and lymphoid cell subsets in the murine inguinal lymph node

FEBS Open Bio, EarlyView.
The circadian system is involved in the temporal regulation of the immune system. Our study reveals that two innate immune populations, NKT cells and neutrophils, predominate at the beginning of the day in healthy mice, highlighting how the time of day influences immune responses.
Paula M. Wagner, César G. Prucca, Lucia Boffelli, Virginia A. Piqueras, Silvia G. Correa, Mariana Maccioni, Mario E. Guido   +6 more
wiley   +1 more source

Biological control and biomass evaluation of Botrytis cinerea : a thesis presented in partial fulfilment of the requirements for the degree of Master of Applied Science at Massey University [PDF]

, 1998
The efficacy of biocontrol agents is often judged by symptom development on inoculated plants. This process can involve long delays, as with Botrytis infection of kiwifruit and an alternative, quicker approach would be useful.
Vingnanasingam, Vallipuram
core  

Dietary nitrate and nitrite protect against doxorubicin‐induced cardiac fibrosis and oxidative protein damage in tumor‐bearing mice

FEBS Open Bio, EarlyView.
Chemotherapies such as doxorubicin can have toxic effects on healthy cardiovascular/heart tissue. Following up on a doxorubicin toxicity study in mice without tumors where nitrate water was cardioprotective (lessened toxicity), this study with tumor‐bearing mice undergoing doxorubicin treatment showed no negative effect of nitrate and nitrite on drug ...
Rama D. Yammani, Xiaofei Chen, Nildris Cruz‐Diaz, Xuewei Zhu, Swati Basu, Daniel B. Kim‐Shapiro, David R. Soto‐Pantoja, Leslie B. Poole   +7 more
wiley   +1 more source

Selected reactive oxygen species and antioxidant enzymes in common bean after Pseudomonas syringae pv. phaseolicola and Botrytis cinerea infection [PDF]

, 2014
Phaseolus vulgaris cv. Korona plants were inoculated with the bacteria Pseudomonas syringae pv. phaseolicola (Psp), necrotrophic fungus Botrytis cinerea (Bc) or with both pathogens sequentially.
A Encina, A Imberty, A Koornneef, A Mika, A Polle, A Sakamoto, A Schouten, AC Maehly, Agata Nowogórska, AM Mayer, B Williamson, BA Adie, BPHJ Thomma, C Beauchamp, C Bowler, C Lamb, DG Mellersh, DJ Capaldi, F Garcia-Arenal, F Passardi, G Creissen, G Oudgenoeg, GJM Beckers, H Derksen, H Thordal-Christensen, HH Flor, I Finiti, J Glazebrook, J Patykowski, J Shi, Jacek Patykowski, JR Alfano, JS Thaler, K Laluk, KG Welinder, M Sedlářová, MC Mehdy, MC Romero-Puertas, MM Bradford, N Doke, N Shlezinger, P Baarlen Van, P Baptista, P Wojtaszek, PP Bolwell, R Goldberg, R Huckelhoven, R Schwacke, RS Dhindsa, S AbuQamar, S AbuQamar, S Dorey, S Takahashi, SJ Burr, T Mengiste, U Takahama, UK Laemmli   +56 more
core   +2 more sources

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

DNA homology within the Rhizobiaceae : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Microbiology at Massey University [PDF]

, 1984
The relationship of rhizobia that nodulate Galega officinalis to the known species of Rhizobium and Bradyrhizobium was investigated. Similarly, the recently discovered fast­ growing soybean nodulating group of rhizobia was studied. Both groups were
Wedlock, David Neil
core  

Effects of elevated [CO2 ] on maize defence against mycotoxigenic Fusarium verticillioides. [PDF]

, 2014
Maize is by quantity the most important C4 cereal crop; however, future climate changes are expected to increase maize susceptibility to mycotoxigenic fungal pathogens and reduce productivity. While rising atmospheric [CO2 ] is a driving force behind the
Alborn, Hans T, Allen, Leon Hartwell, Christensen, Shawn, Dafoe, Nicole J, Huffaker, Alisa, Martins, Vitor F, Romero, Maritza, Schmelz, Eric A, Sims, James, Swerbilow, Jay, Teal, Peter EA, Vaughan, Martha M   +11 more
core   +2 more sources

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source