Forward and Reverse Genetics of B Cell Malignancies: From Insertional Mutagenesis to CRISPR-Cas [PDF]
Frontiers in Immunology, 2021 Cancer genome sequencing has identified dozens of mutations with a putative role in lymphomagenesis and leukemogenesis. Validation of driver mutations responsible for B cell neoplasms is complicated by the volume of mutations worthy of investigation and ...
Joanna C. Dawes +3 more
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Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve [PDF]
Frontiers in Microbiology, 2021 Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems.
Emily C. Hoedt +14 more
doaj +2 more sources
Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics [PDF]
Plants, 2019 Insertional mutagenesis is an indispensable tool for engendering a mutant population using exogenous DNA as the mutagen. The advancement in the next-generation sequencing platform has allowed for faster screening and analysis of generated mutated ...
Hasthi Ram +6 more
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Development of mPing‐based activation tags for crop insertional mutagenesis [PDF]
Plant Direct, 2021 Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits.
Alexander Johnson +12 more
doaj +2 more sources
Insertional mutagenesis in the zoonotic pathogen Chlamydia caviae. [PDF]
PLoS ONE, 2019 The ability to introduce targeted genetic modifications in microbial genomes has revolutionized our ability to study the role and mode of action of individual bacterial virulence factors.
Kimberly Filcek +6 more
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Targeted insertional mutagenesis libraries for deep domain insertion profiling. [PDF]
Nucleic Acids Res, 2020 AbstractDomain recombination is a key principle in protein evolution and protein engineering, but inserting a donor domain into every position of a target protein is not easily experimentally accessible. Most contemporary domain insertion profiling approaches rely on DNA transposons, which are constrained by sequence bias.
Coyote-Maestas W +4 more
europepmc +7 more sources
Long-distance effects of insertional mutagenesis. [PDF]
PLoS ONE, 2011 Most common systems of genetic engineering of mammalian cells are associated with insertional mutagenesis of the modified cells. Insertional mutagenesis is also a popular approach to generate random alterations for gene discovery projects.
Ruchi Singhal +3 more
doaj +4 more sources
Random Insertional Mutagenesis of a Serotype 2 Dengue Virus Clone [PDF]
Bio-Protocol, 2018 Protein tagging is a powerful method of investigating protein function. However, modifying positive-strand RNA virus proteins in the context of viral infection can be particularly difficult as their compact genomes and multifunctional proteins mean even ...
Jeffrey Perry, Andrew Tai
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TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii. [PDF]
PLoS ONE, 2020 Generation and subsequent analysis of mutants is critical to understanding the functions of genes and proteins. Here we describe TIM, an efficient, cost-effective, CRISPR-based targeted insertional mutagenesis method for the model organism Chlamydomonas ...
Tyler Picariello +6 more
doaj +2 more sources
Insertional Mutagenesis of Chlamydomonas reinhardtii
Bio-Protocol, 2015 The unicellular microalga Chlamydomonas reinhardtii (C. reinhardtii) has been used as a reference model for numerous fields of research. Principle research areas are eukaryotic flagellar structure and function, basal bodies (centrioles), cell-cell ...
Chia-Hong Tsai, Christoph Benning
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