Insertional Mutagenesis Identifies a STAT3/Arid1b/β-catenin Pathway Driving Neurofibroma Initiation [PDF]
Cell Reports, 2016 To identify genes and signaling pathways that initiate Neurofibromatosis type 1 (NF1) neurofibromas, we used unbiased insertional mutagenesis screening, mouse models, and molecular analyses.
Jianqiang Wu +22 more
doaj +2 more sources
Generation of an Enhancer-Trapping Vector for Insertional Mutagenesis in Zebrafish. [PDF]
PLoS ONE, 2015 Enhancer trapping (ET) is a powerful approach to establish tissue- or cell-specific reporters and identify expression patterns of uncharacterized genes. Although a number of enhancer-trapping vectors have been developed and a large library of fish lines ...
Chunyan Liu +5 more
doaj +2 more sources
Cancer gene discovery: exploiting insertional mutagenesis. [PDF]
Mol Cancer Res, 2013 Abstract Insertional mutagenesis has been used as a functional forward genetics screen for the identification of novel genes involved in the pathogenesis of human cancers. Different insertional mutagens have been successfully used to reveal new cancer genes.
Ranzani M +3 more
europepmc +4 more sources
Insertional mutagenesis in zebrafish [PDF]
Developmental Dynamics, 2003 AbstractInsertional mutagenesis is a method for identifying genes essential for a given biological process by using the integration of DNA as the mutagen, thereby facilitating the cloning of the mutated gene. The use of retrovirus‐mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes ...
Adam Amsterdam, Nancy Hopkins
openaire +3 more sources
Deregulated Nras expression in knock-in animals harboring a gammaretroviral long terminal repeat at the Nras/Csde1 locus. [PDF]
PLoS ONE, 2013 To investigate mechanisms and phenotypic effects of insertional mutagenesis by gammaretroviruses, we have developed mouse lines containing a single Akv 1-99 long terminal repeat (LTR) and a floxed PGK/Tn5 neomycin cassette at the Nras proto-oncogene at ...
Borja Ballarín-González +5 more
doaj +1 more source
Insertional mutagenesis strategies in zebrafish [PDF]
Genome Biology, 2007 We review here some recent developments in the field of insertional mutagenesis in zebrafish. We highlight the advantages and limitations of the rich body of retroviral methodologies, and we focus on the mechanisms and concepts of new transposon-based mutagenesis approaches under development, including prospects for conditional 'gene trapping' and ...
Sivasubbu, Sridhar +3 more
openaire +3 more sources
Transposon Insertional Mutagenesis in Rice, [PDF]
Plant Physiology, 2001 Transposon mutagenesis systems are being developed in rice to address gene functions using forward and reverse genetics strategies. Although the well-characterized maize ( Zea mays ) transposons seem an obvious choice to develop efficient transposon-tagging systems for gene knockouts and ...
Greco, R. +9 more
openaire +5 more sources
In vivo insertion pool sequencing identifies virulence factors in a complex fungal-host interaction. [PDF]
PLoS Biology, 2018 Large-scale insertional mutagenesis screens can be powerful genome-wide tools if they are streamlined with efficient downstream analysis, which is a serious bottleneck in complex biological systems.
Simon Uhse +5 more
doaj +1 more source
A comprehensive functional map of the hepatitis C virus genome provides a resource for probing viral proteins. [PDF]
, 2014 UnlabelledPairing high-throughput sequencing technologies with high-throughput mutagenesis enables genome-wide investigations of pathogenic organisms. Knowledge of the specific functions of protein domains encoded by the genome of the hepatitis C virus ...
Arumugaswami, Vaithilingaraja +14 more
core +2 more sources
Mutagenesis by retroviral insertion in chemical mutagen-generated quasi-haploid mammalian cells
BioTechniques, 2007 Diploidy is a major obstacle to the mutagenic analysis of function in cultured mammalian cells. Here, we show that 6–8 rounds of chemical mutagenesis generates quasi-haploid cells that can be used as targets for insertional mutagenesis using a specially ...
Sung O. Kim +5 more
doaj +1 more source