Results 41 to 50 of about 45,101 (293)

Gene editing enables T-cell engineering to redirect antigen specificity for potent tumor rejection

Life Science Alliance, 2019
Targeted integration of a tumor-reactive T-cell receptor into the TRAC locus using CRISPR-Cas9 and AAV6 redirects primary human T cells against tumor cells in vitro and in vivo .
Julian J Albers, Tim Ammon, Dario Gosmann, Stefan Audehm, Silvia Thoene, Christof Winter, Ramona Secci, Anja Wolf, Anja Stelzl, Katja Steiger, Jürgen Ruland, Florian Bassermann, Christian Kupatt, Martina Anton, Angela M Krackhardt   +14 more
doaj   +1 more source

Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps [PDF]

, 2009
Background Given its sequenced genome and efficient systemic RNA interference response, the red flour beetle Tribolium castaneum is a model organism well suited for reverse genetics.
Jochen Trauner, Johannes Schinko, Marcé D Lorenzen, Teresa D Shippy, Ernst A Wimmer, Richard W Beeman, Martin Klingler, Gregor Bucher, Susan J Brown   +8 more
core   +1 more source

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

HaSAPPy: A tool for candidate identification in pooled forward genetic screens of haploid mammalian cells.

PLoS Computational Biology, 2018
Haploid cells are increasingly used for screening of complex pathways in animal genomes. Hemizygous mutations introduced through viral insertional mutagenesis can be directly selected for phenotypic changes.
Giulio Di Minin, Andreas Postlmayr, Anton Wutz   +2 more
doaj   +1 more source

Enhanced gene trapping in mouse embryonic stem cells [PDF]

, 2008
Gene trapping is used to introduce insertional mutations into genes of mouse embryonic stem cells (ESCs). It is performed with gene trap vectors that simultaneously mutate and report the expression of the endogenous gene at the site of insertion and ...
Schnütgen, Frank, Hansen, Jens, De-Zolt, Silke, Horn, Carsten, Lutz, Marcus, Floss, Thomas, Wurst, Wolfgang, Ruiz Noppinger, Patricia, Melchner, Harald von (Prof. Dr.)   +8 more
core   +1 more source

AAV and Insertional Mutagenesis

Molecular Therapy, 2007
In this issue of Molecular Therapy, David Russell provides an invited commentary on data presented at the tenth annual meeting of the American Society of Gene Therapy that strongly suggested that adenovirus-associated virus (AAV) vector insertion could in certain models be linked to the development of cancer.1 A short report describing the data that ...
openaire   +2 more sources

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

L1 Retrotransposon Heterogeneity in Ovarian Tumor Cell Evolution

Cell Reports, 2018
Summary: LINE-1 (L1) retrotransposons are a source of insertional mutagenesis in tumor cells. However, the clinical significance of L1 mobilization during tumorigenesis remains unclear.
Thu H.M. Nguyen, Patricia E. Carreira, Francisco J. Sanchez-Luque, Stephanie N. Schauer, Allister C. Fagg, Sandra R. Richardson, Claire M. Davies, J. Samuel Jesuadian, Marie-Jeanne H.C. Kempen, Robin-Lee Troskie, Cini James, Elizabeth A. Beaven, Tristan P. Wallis, Jermaine I.G. Coward, Naven P. Chetty, Alexander J. Crandon, Deon J. Venter, Jane E. Armes, Lewis C. Perrin, John D. Hooper, Adam D. Ewing, Kyle R. Upton, Geoffrey J. Faulkner   +22 more
doaj   +1 more source

Detecting statistically significant common insertion sites in retroviral insertional mutagenesis screens. [PDF]

PLoS Computational Biology, 2006
Retroviral insertional mutagenesis screens, which identify genes involved in tumor development in mice, have yielded a substantial number of retroviral integration sites, and this number is expected to grow substantially due to the introduction of high ...
Jeroen de Ridder, Anthony Uren, Jaap Kool, Marcel Reinders, Lodewyk Wessels   +4 more
doaj   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source