Results 61 to 70 of about 45,101 (293)

Nonmycorrhizal (Myc¯) Mutants of Hebeloma cylindrosporum Obtained Through Insertional Mutagenesis

Molecular Plant-Microbe Interactions, 2004
Polyethylene glycol-mediated transformation of protoplasts was used as a method for insertional mutagenesis to obtain mutants of the ectomycorrhizal fungus Hebeloma cylindrosporum impaired in symbiotic ability.
Jean-Philippe Combier, Delphine Melayah, Colette Raffier, Régis Pépin, Roland Marmeisse, Gilles Gay   +5 more
doaj   +1 more source

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications

Advanced Science, EarlyView.
Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, Raymond J. Monnat, Ross C. Wilson, Benjamin S. Freedman   +12 more
wiley   +1 more source

Phenotypic Screens Identify Genetic Factors Associated with Gametocyte Development in the Human Malaria Parasite Plasmodium falciparum

Microbiology Spectrum, 2023
Transmission of the deadly malaria parasite Plasmodium falciparum from humans to mosquitoes is achieved by specialized intraerythrocytic sexual forms called gametocytes.
Jyotsna Chawla, Ilana Goldowitz, Jenna Oberstaller, Min Zhang, Camilla Valente Pires, Francesca Navarro, Lauriane Sollelis, Chengqi C. Q. Wang, Andreas Seyfang, Jeffrey Dvorin, Thomas D. Otto, Julian C. Rayner, Matthias Marti, John H. Adams   +13 more
doaj   +1 more source

Medicago truncatula functional genomics: an invaluable resource for studies on agriculture sustainability [PDF]

, 2012
Legume functional genomics has moved many steps forward in the last two decades thanks to the improvement of genomics technologies and to the efforts of the research community.
Calderini, Ornella, Panara, Francesco, Porceddu, Andrea   +2 more
core  

Retroviral-mediated Insertional Mutagenesis in Zebrafish [PDF]

, 2011
Since the initial publication of this chapter in 2004, additional methodologies have been developed which could improve and/or complement the original retroviral-mediated insertional mutagenesis. Retroviral vectors have also been shown to be useful for goals other than mutagenesis.
Adam, Amsterdam, Gaurav Kumar, Varshney, Shawn Michael, Burgess   +2 more
openaire   +2 more sources

Temporal and Cell‐Specific Regulation of Synaptic Homeostasis by the Chromatin Remodeler Chd1

Advanced Science, EarlyView.
Chd1, the Drosophila homologue of mammalian CHD2 ‐ a gene linked to autism, epilepsy, and intellectual disability, is required for synaptic homeostatic plasticity. Chd1 in glia is necessary for the rapid induction of synaptic homeostasis, whereas Chd1 in motoneurons, muscle, and glia is critical for long‐term maintenance.
Danielle T. Morency, Tao Cui, Yimei Cai, Chloe Lok, Rachel E. Nokku, Ruoxian Huang, Grace L. Chu, Yumeng Xie, Saleem W. Abu‐Tayeh, Kaikai He, Chengjie Qiu, Junyi Wang, Paxton M. Paganelli, Ting Wang, Gabrielle Williams, Sreejith Nair, Huadong Pei, Dion K. Dickman, Stefano Vicini, Tingting Wang   +19 more
wiley   +1 more source

Modeling integration site data for safety assessment with MELISSA

Nature Communications
Gene and cell therapies pose safety concerns due to potential insertional mutagenesis by viral vectors. We introduce MELISSA, a regression-based statistical framework for analyzing Integration Site (IS) data to assess insertional mutagenesis risk, by ...
Tsai-Yu Lin, Giacomo Ceoldo, Kimberley House, Matthew Welty, Thao Thi Dang, Denise Klatt, Christian Brendel, Michael P. Murphy, Kenneth Cornetta, Danilo Pellin   +9 more
doaj   +1 more source

Retroviral insertional mutagenesis identifies oncogene cooperation [PDF]

Cancer Science, 2005
Retroviral insertional mutagenesis has been applied to identify oncogenes that are important for both human and rodent carcinogenesis. The method reveals not only primary oncogenes but also cooperative genes that might be affected as second or third hits in multistep carcinogenesis.
openaire   +2 more sources

Divergent Regulatory Effects of Jasmonic Acid on Tomato Lycopene Biosynthesis Under Light and Dark Conditions

Advanced Science, EarlyView.
This study reveals the mechanism by which jasmonic acid (JA) regulates lycopene synthesis under light and dark conditions. In light, JA activates SlMYC2, which suppresses SlPIF1a and promotes SlPSY1 expression. In darkness, JA induces the acetyltransferase SlNATA1, which acetylates the dark‐accumulated SlPIF1a, thereby repressing SlPSY1 expression ...
Jiayi Xu, Lingfeng He, Jialong Zhang, Hao Cui, Hongxin Li, Haijun Zhang, Jiaojiao Zhang, Lun Liu, Kunpeng Xu, Yang‐Dong Guo, Na Zhang   +10 more
wiley   +1 more source

A novel approach to identify driver genes involved in androgen-independent prostate cancer [PDF]

, 2014
Background Insertional mutagenesis screens have been used with great success to identify oncogenes and tumor suppressor genes. Typically, these screens use gammaretroviruses (γRV) or transposons as insertional mutagens.
Bii, Victor, Meadows, Gary G, Nalla, Arun, Rae, Dustin T, Schinke, Ellyn N, Tedrick, Laura, Trobridge, Grant D   +6 more
core   +2 more sources