Results 71 to 80 of about 45,101 (293)
Advanced Science, EarlyView.This study presents the first telomere‐to‐telomere genomes and population resources for Diaporthe pathogens, uncovering the largest known fungal repertoire of secondary metabolite gene clusters. Structural variations and horizontal gene transfer drive cluster diversification, while specific rapidly evolving clusters control virulence, offering novel ...
Kainan Li +9 more
wiley +1 more source
FSTVAL: a new web tool to validate bulk flanking sequence tags
Plant Methods, 2012 Background Information about a transgene locus is one of the major concerns in transgenic research because expression of the transgene or a gene interrupted by the integration event could be affected. Thus, the flanking sequences obtained from transgenic
Kim Joung +11 more
doaj +1 more source
Light controls growth and development via a conserved pathway in the fungal kingdom. [PDF]
PLoS Biology, 2005 Light inhibits mating and haploid fruiting of the human fungal pathogen Cryptococcus neoformans, but the mechanisms involved were unknown. Two genes controlling light responses were discovered through candidate gene and insertional mutagenesis approaches.
Alexander Idnurm, Joseph Heitman
doaj +1 more source
, 2016 Background: Knowledge of the intrinsic properties of embryonic stem cells is essential before the possibility of using these cells for therapeutic purposes becomes a reality. Insertional mutagenesis screens are widely used to identify genes sufficient to
Lee, Sung-Hun, Rangasamy, Danny
core +1 more source
The expanding universe of transposon technologies for gene and cell engineering [PDF]
, 2010 Transposable elements can be viewed as natural DNA transfer vehicles that, similar to integrating viruses, are capable of efficient genomic insertion.
Zoltán Ivics, Zsuzsanna Izsvák
core +4 more sources
Advanced Science, EarlyView.This study discovered a new pathway that tells fruit flies when to stop eating. It found that rising blood sugar (fructose) is detected by a sensor called GR43a. This triggers a chain reaction involving the satiety signal sulfakinin and its receptor, ultimately activating a final satiety signal, ILP5.
Hong‐Fei Li +7 more
wiley +1 more source
Conditional ablation of the RFX4 isoform 1 transcription factor: Allele dosage effects on brain phenotype. [PDF]
PLoS ONE, 2018 Regulatory factor X4 (RFX4) isoform 1 is a recently discovered isoform of the winged helix transcription factor RFX4, which can bind to X-box consensus sequences that are enriched in the promoters of cilia-related genes.
Ping Xu +6 more
doaj +1 more source
Respiratory-deficient mutants of the unicellular green alga Chlamydomonas: A review. [PDF]
, 2013 Genetic manipulation of the unicellular green alga Chlamydomonas reinhardtii is straightforward. Nuclear genes can be interrupted by insertional mutagenesis or targeted by RNA interference whereas random or site-directed mutagenesis allows the ...
Cardol, Pierre +4 more
core +1 more source
Advanced Science, EarlyView.Microproteins are hidden treasures encoded by the “dark proteome” but remain largely underexplored due to the lack of highly efficient tools. We developed a molecularly imprinted polymers (MIPs)‐based toolbox (CLAIMID) to achieve accelerated and ultrasensitive microproteins validation at multiple biological scales (single living cells, cell populations,
Hui He +10 more
wiley +1 more source
Transducin beta-like gene FTL1 is essential for pathogenesis in Fusarium graminearum [PDF]
, 2009 Fusarium head blight caused by Fusarium graminearum is an important disease of wheat and barley. In a previous study, we identified several mutants with reduced virulence by insertional mutagenesis. A transducin beta-like gene named FTL1 was disrupted in
Ding, S.L. +7 more
core +2 more sources