Results 91 to 100 of about 3,727,616 (304)
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Instructional Development (1994 Winter Quarter)
, 1994 University of Minnesota, Duluth. Instructional Development Service. (1994). Instructional Development (1994 Winter Quarter).
University of Minnesota, Duluth. Instructional Development Service
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Instructional Development (2007 Spring Semester) [PDF]
, 2007 University of Minnesota Duluth. Instructional Development Service. (2007). Instructional Development (2007 Spring Semester).
University of Minnesota Duluth. Instructional Development Service
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Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
PROPOSAL FOR A MOOC INSTRUCTIONAL DESIGN BASED ON THE THEORY DEVELOPMENT AND MASTERY LEARNIG
Ra Ximhai, 2016 The first massive open online courses cMOOC lacked an instructional design and have now shifted to models that have an instructional design called xMOOC.
José Emilio Sánchez-García +2 more
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Jurnal Edutech UndikshaUniversities need to have an educational focus by using a multicampus system that must be integrated to achieve its goals. Therefore, an instructional communication strategy needs to be implemented to support management in building communication.
Laskmi Dewi +4 more
doaj +1 more source
Instructional Development (2011 Fall Semester)
, 2011 University of Minnesota Duluth. Instructional Development Service. (2011). Instructional Development (2011 Fall Semester).
University of Minnesota Duluth. Instructional Development Service
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Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Instructional Development (2011 Spring Semester)
, 2011 University of Minnesota Duluth. Instructional Development Service. (2011). Instructional Development (2011 Spring Semester).
University of Minnesota Duluth. Instructional Development Service
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