Results 91 to 100 of about 166,808,572 (290)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Cognitive dysfunction, social participation, and performance of activities of daily living among a sub-urban community-dwelling older adults in Nigeria

Cogent Gerontology
Cognitive dysfunction (CF) in older adults significantly impacts activities of daily living (ADLs), social participation (SP), instrumental activities of daily living (IADLs), and overall quality of life. This cross-sectional study assessed CF, SP, ADLs,
Obiekwe Sochima Johnmark, Uzoamaka Nwakego Akobundu, Okwudili Precious Emuka, Akosile Christopher Olusanjo, Uchechukwu Martha Chukwuemeka, Ukamaka Gloria Mgbeojedo, Uchenna Prosper Okonkwo   +6 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Associations of self- and informant-reported functional impairment with cognitive performance and incident dementia

The Journal of Prevention of Alzheimer's Disease
Introduction: Functional impairment is central to dementia diagnosis, typically assessed through basic and instrumental activities of daily living (ADLs/IADLs) reported by participants or informants.
Yaqing Gao, Paola Zaninotto, Andrew Steptoe   +2 more
doaj   +1 more source

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

Proposal for a revised Barthel index classification based on mortality risk assessment in functional dependence for basic activities of daily living

Frontiers in Public Health
IntroductionFunctional dependence on the performance of basic activities of daily living (ADLs) is associated with increased mortality. In this study, the Barthel index and its activities discriminate long-term mortality risk, and whether changes in this
Vicente Martín Moreno, Vicente Martín Moreno, María Inmaculada Martínez Sanz, Amanda Martín Fernández, Sara Guerra Maroto, Eva Sevillano Fuentes, Elena Pérez Rico, Irene Sánchez González, Miriam Fernández Gallardo, Julia Herranz Hernando, María Palma Benítez Calderón, Laura Calderón Jiménez, Elena Sánchez Rodríguez, Miguel Recuero Vázquez, Helena Alonso Samperiz, Irene León Saiz, Juana Marcos Guerra   +16 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

The impact of a residential fire on occupational engagement and performance: A reflexive narrative approach

Burns Open
Residential fires are a devastating and stressful life event. The high number of residential fires per year and significant associated stressors impacts all aspects of daily life.
Gregory Chown
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Association between the different basic activities of daily living on the Barthel index and community living, use of mobility aids, and the survival at 5 years

Frontiers in Public Health
IntroductionThe Barthel index has designed to assess functional capacity to perform basic activities of daily living. This study analyzes the association between each of these activities and community living, the use of mobility aids, and various ...
Vicente Martín Moreno, Elena Pérez Rico, Amanda Martín Fernández, Jorge Hurtado Gallar, Irene Sánchez González, Elena Sánchez Rodríguez, María Inmaculada Martínez Sanz, Miriam Fernández Gallardo, Juana Marcos Guerra, María del Carmen Cubero Sobrados, Laura Flórez Busto, Asunción García Mateos, Beatriz Alonso Serna, Annly Luzmila Vásquez Alay   +13 more
doaj   +1 more source