Results 311 to 320 of about 98,089 (330)

β-Cell Lipases and Insulin Secretion

Diabetes, 2006
Lipids have been implicated in β-cell stimulus-secretion coupling. Thus, lipases in β-cells would be required to generate coupling factors from intracellular lipids. Indeed, we found that glucose stimulates lipolysis in rodent islets and clonal β-cells.
Malin Fex, Stephanie Lucas, Maria Sörhede Winzell, Bo Ahrén, Cecilia Holm, Hindrik Mulder   +5 more
openaire   +1 more source

Hexose Recognition by Insulin-Secreting BRIN-BD11 Cells

Biochemical and Biophysical Research Communications, 1996
Clonal BRIN-BD11 cells were produced by electrofusion of NEDH rat islet B-cells with immortal RINm5F cells. Western blotting analysis revealed that unlike RINm5F, novel BRIN-BD11 cells expressed high levels of the glucose transport protein GLUT-2, coupled with a rapid and sustained uptake of D-glucose, significantly greater than RINm5F after only 5 min
N H, McClenaghan, A M, Gray, C R, Barnett, P R, Flatt   +3 more
openaire   +2 more sources

Insulin Secreting Islet Cell Tumors of the Pancreas

Archives of Surgery, 1966
FUNCTIONING islet tumors of the pancreas producing spontaneous hypoglycemia have been studied with interest at McGill University since the original report from the Pathological Institute by Nicholls 1 in 1902. In 1927, Wilder et al 2 demonstrated the relationship between hyperinsulinism and a malignant islet cell tumor by means of bioassay of the ...
J J, White, V A, Piccone, D R, Webster, J R, McCorriston   +3 more
openaire   +2 more sources

Bioartificial Pancreas: Alternative Supply of Insulin‐secreting Cells

Annals of the New York Academy of Sciences, 1999
ABSTRACT: In this study, insulin secretion function of INS‐1 cells immunoisolated in microcapsules was evaluated. Following encapsulation, the immunoisolated INS‐1 cells continued to propagate and flourish within the microcapsules during the entire two‐month in vitro incubation period.
D, Zhou, E, Kintsourashvili, S, Mamujee, I, Vacek, A M, Sun   +4 more
openaire   +2 more sources

B-cell size influences glucose-stimulated insulin secretion

American Journal of Physiology-Cell Physiology, 1993
To determine whether the heterogeneous B-cell response to glucose is related to a different metabolic handling of this sugar, we have compared rat B-cells differing in their redox response to glucose stimulation. To this end, a population of B-cells showing increased NAD(P)H autofluorescence after a 15-min exposure to 16.7 mM glucose was sorted from a
Giordano, E., Cirulli, V., Bosco, Domenico, Rouiller, Dominique, Halban, Philippe A., Meda, Paolo   +5 more
openaire   +3 more sources

Potassium channels of the insulin‐secreting B cell

Fundamental & Clinical Pharmacology, 1992
Summary—Ionic and electrical events play a central role in the stimulus‐secretion coupling of the pancreatic B cell. Potassium permeability is critically involved in the regulation of B cell membrane potential and insulin secretion. In the absence of glucose, membrane potential remains stable, around − 65 mV. This resting potential is mainly determined
P, Petit, M M, Loubatières-Mariani
openaire   +2 more sources

Engineering cultured insulin-secreting pancreatic B-cell lines

Journal of Molecular Medicine, 1999
Despite many triumphs, a significant limitation of the usefulness of many of the available B-cell lines for the study of insulin secretion are either inappropriate or lack of responsiveness to glucose. Commonly employed cell lines generated prior to the 1990s following X-ray irradiation (RINm5F cells) or simian virus 40 B-cell transformation (HIT-T15 ...
N H, McClenaghan, P R, Flatt
openaire   +2 more sources

Inhibition of K-Channels in Insulin Secreting Cells

1986
The major physiological stimulus for insulin secretion from pancreatic β-cells is an elevation of the plasma glucose concentration. The initial effect is to evoke membrane depolarization4 followed by a cyclical pattern of electrical activity consisting of slow waves of depolarization with action potential-like spikes.9,12 Tracer flux studies have ...
O H, Petersen, I, Findlay, M J, Dunne
openaire   +2 more sources

Transcription factor AP-2β inhibits glucose-induced insulin secretion in cultured insulin-secreting cell-line

Diabetes Research and Clinical Practice, 2009
We previously identified the transcription factor activating enhancer-binding protein-2beta (AP-2beta) gene as a new candidate for conferring susceptibility to type 2 diabetes. To ascertain the possible involvement of AP-2beta in the pathogenesis of type 2 diabetes we examined the effects of AP-2beta on glucose-induced insulin secretion.We measured the
Shuichi, Tsukada, Masa-Aki, Kobayashi, Shintaro, Omori, Hiroyuki, Unoki, Shiro, Maeda   +4 more
openaire   +2 more sources

Insulin‐Secreting Islet‐Cell Tumours of the Pancreas

Australian and New Zealand Journal of Surgery, 1967
Some current thoughts on the subject of insulin‐secreting islet‐cell tumours of the pancreas are presented, and a typical case is reported. On the diagnostic side, mention is made of newer aids to diagnosis in the form of tolbutamide and leucine tests and possibly insulin assays.
openaire   +2 more sources