Results 91 to 100 of about 1,117,145 (290)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Comportamento ingestivo e digestão de nutrientes em bovinos de corte alimentados com silagem de capim = Intake behavior and nutrient digestion of beef cattle fed with grass silage

open access: yesActa Scientiarum: Animal Sciences, 2009
Avaliou-se o efeito do teor de matéria seca, uso de inoculante bacteriano e a redução do tamanho de partículas da silagem de capim-Tanzânia (Panicum maximum), sobre os parâmetros digestivos.
Daniele Rebouças Santana Loures   +11 more
doaj  

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

The Use of Mesquite Pods (Prosopis spp.) as an Alternative to Improve the Productive Performance and Methane Mitigation in Small Ruminants: A Meta-Analysis

open access: yesFermentation
Mesquite (Prosopis spp.), a highly nutritious legume from arid regions characterized by its secondary metabolites, offers a cost-effective resource to provide energy and protein for small ruminant farmers in harsh environments.
Juan Carlos Angeles-Hernandez   +9 more
doaj   +1 more source

Increased Fiber Intake Results in Better Overall Survival and Lower GI-aGVHD in Allo-HCT Recipients and Pre-Clinical Gvhd Models [PDF]

open access: bronze
Jenny Paredes   +17 more
openalex   +1 more source

Immunoprotective effects of oral intake of heat-killed Lactobacillus pentosus strain b240 in elderly adults: a randomised, double-blind, placebo-controlled trial – CORRIGENDUM [PDF]

open access: bronze, 2016
Shoji Shinkai   +15 more
openalex   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

open access: yesFEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu   +10 more
wiley   +1 more source

Níveis de uréia em suplementos para terminação de bovinos em pastejo durante a época seca = Urea levels in supplements for finishing cattle at pasture during the dry season

open access: yesActa Scientiarum: Animal Sciences, 2007
Avaliaram-se níveis de inclusão de uréia em suplementos sobre o consumo, a digestibilidade e parâmetros do metabolismo dos compostos nitrogenados em bovinos, manejados em pastagem de Brachiaria brizantha cv. Marandu, no período de seca.
Tiago Sabella Acedo   +5 more
doaj  

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