Results 141 to 150 of about 6,332,703 (315)
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
United States Bureau of Mines Bulletin 486
Report published by the U.S. Bureau of Mines containing the results of a survey of the natural gas resources available in the United States. From the summary:"The Results of a Bureau of Mines survey of helium resources are given in this report and ...
Anderson, C. C. +2 more
core
Study of density of interface states in MOS structure with ultrathin NAOS oxide
Jurečka Stanislav +4 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
National Cyber Strategy of the United States of America
With the release of this National Cyber Strategy, the United States now has its first fully articulated cyber strategy in 15 years. This strategy explains how the US will: Defend the homeland by protecting networks, systems, functions, and data; Promote ...
United States. White House Office.
core
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti +5 more
wiley +1 more source
The United States We Will Live In
Front: The text highlights what happened in the first weeks of the occupation of Japan. Photographs: General MacArthur receives Emperor Hirohito at the U.S.
[United States.] Army Information Branch.
core
Spin relaxation of a donor electron coupled to interface states. [PDF]
Huang P, Bryant GW.
europepmc +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source

