Results 221 to 230 of about 81,393 (255)

The mouse interleukin 1 receptor antagonist protein: gene structure and regulation in vitro

Cytokine, 1994
The interleukin 1 receptor antagonist (IL-1ra) protein is an inhibitor of the pro-inflammatory cytokine interleukin 1. We have sequenced the mouse gene encoding the monocyte form of IL-1ra (IL-1rn) and compared it with the sequence of the human homologue.
K A, Zahedi, C M, Uhlar, M, Rits, A E, Prada, A S, Whitehead   +4 more
openaire   +2 more sources

Interleukin-1 receptor antagonist prevents sepsis-induced inhibition of protein synthesis

American Journal of Physiology-Endocrinology and Metabolism, 1994
To understand the role of interleukin-1 (IL-1) as a mediator of the sepsis-induced skeletal muscle catabolism, we investigated the effects of a specific IL-1 receptor antagonist (IL-1ra) on skeletal muscle protein metabolism in a rodent model of chronic abdominal sepsis.
R, Cooney, E, Owens, C, Jurasinski, K, Gray, J, Vannice, T, Vary   +5 more
openaire   +2 more sources

Initial crystallographic analysis of a recombinant human interleukin-1 receptor antagonist protein

Acta Crystallographica Section D Biological Crystallography, 1994
We report the crystallization of samples of a recombinant preparation of human interleukin-1 receptor antagonist protein (IRAP) and solution of the crystal structure by isomorphous replacement methods. Crystals were obtained by the hanging-drop vapor-diffusion method at 277 K from solutions of PEG 4000 containing sodium chloride, dithiothreitol and ...
L L, Clancy, B C, Finzel, A W, Yem, M R, Deibel, N A, Strakalaitis, D P, Brunner, R M, Sweet, H M, Einspahr   +7 more
openaire   +2 more sources

Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein

Nature, 1990
A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced. A complementary DNA coding for IRAP was cloned and sequenced. The mature translation product of the cDNA has been expressed in Escherichia coli and was an active competitive inhibitor of the binding of ...
D. B. Carter, M. R. Deibel, C. J. Dunn, C.-S. C. Tomich, A. L. Laborde, J. L. Slightom, A. E. Berger, M. J. Bienkowski, F. F. Sun, R. N. McEwan, P. K. W. Harris, A. W. Yem, G. A. Waszak, J. G. Chosay, L. C. Sieu, M. M. Hardee, H. A. Zurcher-Neely, I. M. Reardon, R. L. Heinrikson, S. E. Truesdell, J. A. Shelly, T. E. Eessalu, B. M. Taylor, D. E. Tracey   +23 more
openaire   +2 more sources

Cloning, heterologous expression and characterization of murine interleukin 1 receptor antagonist protein

European Journal of Immunology, 1991
AbstractA cDNA coding for the human interleukin 1 receptor antagonist protein (IL 1Ra) was used to clone the corresponding murine cDNA. The nucleotide sequence of the open reading frame coding for the processed form of mIL 1Ra predicted a 152‐residue protein that was 77% identical to human IL 1Ra.
M E, Shuck, T E, Eessalu, D E, Tracey, M J, Bienkowski   +3 more
openaire   +2 more sources

Genetic Regulation and Activities of an Interleukin 1 Receptor Antagonist Protein

1993
Publisher Summary The cytokine interleukin 1 (IL-1) plays an important role in the response to infection and injury. IL-1 can have positive effects on events in the immune response, including T, B, and natural killer (NK) cell activation. Glucocorticoids inhibit both the synthesis of IL-1 and its action on glucocorticoid-sensitive IL-1-responsive ...
Donald B. Carter, Ann E. Berger, Daniel E. Tracey   +2 more
openaire   +1 more source

Potential agonist action of the interleukin-1 receptor antagonist protein: implications for treatment of women.

The Journal of Clinical Endocrinology & Metabolism, 1993
The interleukin-1 receptor antagonist protein (IRAP) caused a concentration-related increase in prostaglandin production by human decidual cells. The degree and time course of this stimulatory action were identical to those induced by interleukin 1 beta (IL-1 beta).
Mitchell, M.D., Edwin, S.S., Silver, R.M., Romero, R.J.   +3 more
openaire   +5 more sources

Interleukin-1α and Interleukin-1β Stimulate Adrenocorticotropin Secretion in the Rat through a Similar Hypothalamic Receptor(s): Effects of Interleukin-1 Receptor Antagonist Protein

Neuroendocrinology, 2008
Numerous reports have demonstrated that interleukin-1 β (IL-1β) is a potent secretagogue for adrenocorticotropin (ACTH) and that IL-1α appears to be considerably less efficacious. To clarify apparent differences in the potency of IL- 1α vs. -β on ACTH secretion from a functional perspective, the IL-1 receptor antagonist protein, IRAP, was utilized ...
S G, Matta, K M, Linner, B M, Sharp
openaire   +2 more sources

Purification of Human Recombinant Interleukin 1 Receptor Antagonist Proteins uponBacillus subtilisSporulation

Protein Expression and Purification, 1997
Human interleukin 1 receptor antagonist (IL-1ra) and IL-1ra mutants were constitutively expressed in recombinant Bacillus subtilis in endocellular and active form. In order to optimize the purification of the recombinant proteins, a new method has been developed.
G, Maurizi, V, Di Cioccio, G, Macchia, P, Bossù, C, Bizzarri, U, Visconti, D, Boraschi, A, Tagliabue, P, Ruggiero   +8 more
openaire   +2 more sources

Recombinant interleukin-1 receptor antagonist protein prevents sensitization and intestinal anaphylaxis in Guinea pigs

Life Sciences, 1993
Recombinant interleukin-1 receptor antagonist protein (rlRAP, 0.5 mg/kg) administered intraperitoneally in guinea pigs one hour before primary and booster parenteral sensitization (1 ml) by cow milk, led to a reduced immunoglobulin E (IgE) production, as displayed by a passive cutaneous anaphylaxis test.
Theodorou, Vassilia, Fioramonti, Jean, Bueno, Lionel   +2 more
openaire   +3 more sources