Results 61 to 70 of about 1,186,479 (304)

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

open access: yesFEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani   +4 more
wiley   +1 more source

Generation of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus Stably Expressing Two Marker Genes

open access: yesFrontiers in Veterinary Science, 2020
Porcine reproductive and respiratory syndrome virus (PRRSV) has been used as a gene expression vector in the development of vaccines. Most of these recombinant PRRSV vectors express only a single foreign gene through either an internal insertion in the ...
Hao Wang   +8 more
doaj   +1 more source

Urinary Neutrophil Gelatinase-associated Lipocalin as a Marker for Identification of Acute Kidney Injury and Recovery in Dogs with Gentamicin-induced Nephrotoxicity. [PDF]

open access: yes, 2016
BackgroundAcute kidney injury (AKI) is associated with high mortality rates in dogs, which may be a consequence of late recognition using traditional diagnostic tests.
Cowgill, LD   +6 more
core   +2 more sources

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

open access: yesFEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Speciation Progress: A Case Study on the Bushcricket Poecilimon veluchianus. [PDF]

open access: yesPLoS ONE, 2015
Different mechanisms such as selection or genetic drift permitted e.g. by geographical isolation can lead to differentiation of populations and could cause subsequent speciation.
Lucienne Eweleit   +2 more
doaj   +1 more source

Time after time – circadian clocks through the lens of oscillator theory

open access: yesFEBS Letters, EarlyView.
Oscillator theory bridges physics and circadian biology. Damped oscillators require external drivers, while limit cycles emerge from delayed feedback and nonlinearities. Coupling enables tissue‐level coherence, and entrainment aligns internal clocks with environmental cues.
Marta del Olmo   +2 more
wiley   +1 more source

Digestibidade e desempenho produtivo em cabritos mestiços ½ Boer x ½ Saanen alimentados com dietas contendo gordura protegida

open access: yesSemina: Ciências Agrárias, 2015
 Objetivou-se com este trabalho, avaliar a ingestão, os parâmetros de desempenho produtivo, a digestibilidade da matéria seca e dos nutrientes de dietas com concentrações crescentes de energia metabolizável contendo gordura protegida, e a análise ...
Sérgio Mangano de Almeida Santos   +7 more
doaj   +1 more source

Segmentation and tracking of video objects for a content-based video indexing context [PDF]

open access: yes, 2000
This paper examines the problem of segmentation and tracking of video objects for content-based information retrieval. Segmentation and tracking of video objects plays an important role in index creation and user request definition steps.
Chassaing, F   +3 more
core   +1 more source

Structural biology of ferritin nanocages

open access: yesFEBS Letters, EarlyView.
Ferritin is a conserved iron‐storage protein that sequesters iron as a ferric mineral core within a nanocage, protecting cells from oxidative damage and maintaining iron homeostasis. This review discusses ferritin biology, structure, and function, and highlights recent cryo‐EM studies revealing mechanisms of ferritinophagy, cellular iron uptake, and ...
Eloise Mastrangelo, Flavio Di Pisa
wiley   +1 more source

Applying an internal transcribed spacer as a single molecular marker to differentiate between Tetraselmis and Chlorella species

open access: yesFrontiers in Microbiology, 2023
In the realm of applied phycology, algal physiology, and biochemistry publications, the absence of proper identification and documentation of microalgae is a common concern.
Wael A. Fathy   +10 more
doaj   +1 more source

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