Internal transcribed spacer dimorphism and diversity in Dientamoeba fragilis.
The internal transcribed spacer (ITS) region of the ribosomal RNA operon is frequently used for detecting sequence variation among closely related species as it is usually homogeneous within strains but evolves more rapidly than ribosomal RNA coding ...
Clark, C Graham+2 more
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Internal transcribed spacer primer evaluation for vascular plant metabarcoding [PDF]
The unprecedented ongoing biodiversity decline necessitates scalable means of monitoring in order to fully understand the underlying causes. DNA metabarcoding has the potential to provide a powerful tool for accurate and rapid biodiversity monitoring. Unfortunately, in many cases, a lack of universal standards undermines the widespread application of ...
Andreas Kolter, Birgit Gemeinholzer
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Evolution of ribosomal internal transcribed spacers in Deuterostomia [PDF]
AbstractSequences of ribosomal internal transcribed spacers (ITSs) are of great importance to molecular phylogenetics and DNA barcoding, but remain unstudied in some large taxa of Deuterostomia. We have analyzed completeITS1andITS2sequences in 62 species from 16 Deuterostomia classes, withITSssequences in 24 species from 11 classes initially obtained ...
Alexander Dyomin+5 more
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Identification of the Fungal Community in Otomycosis by Internal Transcribed Spacer Sequencing
We used internal transcribed spacer (ITS) sequencing to identify the fungal community in otomycosis patients and to evaluate the treatment effects of bifonazole. Ten patients who visited the Department of Otolaryngology of Jiangsu Provincial Hospital on Integration of Chinese and Western Medicine from May 2020 to April 2021 were recruited.
Xiaona Gu+5 more
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Extensive intragenomic variation in the internal transcribed spacer region of fungi
Fungi are among the most biodiverse organisms in the world. Accurate species identification is imperative for studies on fungal ecology and evolution. The internal transcribed spacer (ITS) rDNA region has been widely accepted as the universal barcode for fungi.
Michael J. Bradshaw+10 more
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Confirmation of taxonomic status of black yeast-like fungus by three gene phylogeny [PDF]
Status of black melanin-containing yeast-like fungus Exophiala alcalophila isolated from microorganism complex of hermetic damaged in conditions of indoor high humidity in Kiev, Ukraine is proved by combined phylogenetic analysis based on sequences of ...
Beregova, T. V.+4 more
core +1 more source
First report of grapevine dieback caused by Lasiodiplodia theobromae and Neoscytalidium dimidiatum in Basrah, Southern Iraq [PDF]
In Basrah, grapevines suffer from dieback. Lasiodiplodia theobromae and Neoscytalidium dimidiatum were isolated from diseased grapevines, Vitis vinifera L.
Al-Badran, Adnan+4 more
core +2 more sources
rDNA internal transcribed spacer sequence analysis of Lycoris Hert. [PDF]
The interspecific relationships of Lycoris species were studied by internal transcribed spacer (ITS) sequences. ITS fragments of 14 species were amplified, sequenced and analysed.
Chen, Dongming+4 more
core +2 more sources
Ribosomal DNA internal transcribed spacer 1 and internal transcribed spacer 2 regions as targets for molecular identification of medically important Zanthoxylum schinifolium [PDF]
Molecular approaches are now being developed to provide a more rapid and objective identification compared to traditional phenotypic methods. Nuclear ribosomal DNA (nrDNA) targets, especially internal transcribed spacer 1 and 2 (ITS1 and ITS2), have been
Oh-Woung, K+3 more
core +2 more sources
Evaluation of internal transcribed spacer 2-RFLP analysis for the identification of dermatophytes [PDF]
A total of 95 isolates, belonging to 33 species of five dermatophyte genera, i.e. Arthroderma (15 species), Chrysosporium (two), Epidermophyton (one), Microsporum (three) and Trichophyton (12), were studied using internal transcribed spacer 2 (ITS2)-PCR-RFLP analysis (ITS2-RFLP), consisting of amplification of the ITS2 region, restriction digestion ...
De Baere, Thierry+4 more
openaire +5 more sources