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Testing the utility of internally transcribed spacer sequences in coral phylogenetics

Molecular Ecology, 2004
AbstractReef‐building corals often possess high levels of intraindividual and intraspecific ribosomal DNA (rDNA) variation that is largely polyphyletic between closely related species. Polyphyletic rDNA phylogenies coupled with high intraindividual rDNA variation have been taken as evidence of introgressive hybridization in corals.
Steven V, Vollmer, Stephen R, Palumbi
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Putative group I introns in the eukaryote nuclear internal transcribed spacers

Current Genetics, 2019
Group I introns are mobile genetic elements that interrupt genes encoding proteins and RNAs. In the rRNA operon, introns can insert in the small subunit (SSU) and large subunit (LSU) of a wide variety of protists and various prokaryotes, but they were never found in the ITS region.
Daniele Corsaro, Danielle Venditti
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16S-23S rDNA internal transcribed spacer regions in four Proteus species

Journal of Microbiological Methods, 2009
Proteus is a Gram-negative, facultative anaerobic bacterium. In this study, 813 Proteus 16S-23S rDNA internal transcribed spacer (ITS) sequences were determined from 46 Proteus strains, including 388 ITS from 22 P. mirabilis strains, 211 ITS from 12 P. vulgaris strains, 169 ITS from 10 P. penneri strains, and 45 ITS from 2 P. myxofaciens strains.
Boyang, Cao   +6 more
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Nuclear rRNA transcript processing versus internal transcribed spacer secondary structure

Trends in Genetics, 2015
rRNA is one of the few universal features of life, making it uniquely suited to assess phylogenetic relationships. The processing of the initial polycistronic rRNA transcript is also a conserved process, involving numerous cleavage events and the generation of secondary structures.
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Internal Transcribed Spacer (ITS)-PCR Identification of MRSA

2007
Polymerase chain reaction (PCR) analysis of the 16S-23S rRNA gene internal transcribed spacer (ITS) followed by microchip gel electrophoresis was useful for identification of staphylococci and for strain delineation of Staphylococcus aureus. In the study presented in this chapter, 74 ITS patterns were demonstrated among 1,188 isolated colonies of S ...
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Restriction polymorphisms in the internal transcribed spacers and 5.8S rDNA ofSaccharomyces

Current Microbiology, 1992
Polymorphisms in enzymatically amplified ribosomal DNA (rDNA) were examined in 18 strains of Saccharomyces. Restriction patterns generated from the region spanning the internal transcribed spacers (ITS) and the 5.8S rDNA produced two clusters corresponding to S. bayanus and S. cerevisiae. The type culture of S.
F I, Molina, T, Inoue, S C, Jong
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Evolutionary divergence of ribosomal internal transcribed spacer 2 in lizards

Molecular Biology, 2006
Internal transcribed spacers 1 and 2 (ITS1 and ITS2) are known to play an important role in rRNA maturation, yet the mechanism of their action is still not completely understood. Comparison of the ITS1 and ITS2 nucleotide sequences for various organisms reveals conserved regions, which are potentially involved in rRNA biogenesis, and yields new ...
A. S. Voronov   +3 more
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[Comparison of rDNA internal transcribed spacer sequences in asparagus].

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 2011
Using ITS sequence of nine species to identify counterfeiting medicine and analyse phylogenetic of Asparagus.Analysing ITS sequences by amplification, cloning,sequencing and alignment.The length range of ITS sequence of nine species was from 711 to 748 bp, the percentage of G + C content was about 60%.
Li-Jun, Ou   +6 more
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Diversity of the internal transcribed spacer of rDNA in morels

Canadian Journal of Microbiology, 1999
The internal transcribed spacer (ITS) of rDNA was analysed by PCR/RFLP in 66 strains belonging to 11 putative species of the Morchellaceae and 3 species of the Discinaceae. For certain taxa, in particular Morchella esculenta, Morchella conica, and Morchella elata, isolates from distant geographical origins were available.
Wipf, Daniel   +4 more
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Using internal transcribed spacers for Calanoida (Crustacea, Copepoda) species identification

Marine Biodiversity, 2018
Mitochondrial gene Cytochrome Oxidase I (COI) is a standard marker for molecular identification of many animal groups, but its general use does not apply for calanoid copepods. This study aimed to test nuclear ribosomal internal transcribed spacer (ITS1 and ITS2) as barcode for this animal group.
Gustavo M. Rocha   +3 more
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