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Authentication of Medicinal Dendrobium Species by the Internal Transcribed Spacer of Ribosomal DNA
Planta Medica, 2001Herba Dendrobii (Shihu) is a commonly used Chinese medicine derived from the stem of several orchid species belonging to the genus Dendrobium. It is rather expensive and adulteration is frequent. Proper authentication of the medicinal species is necessary to protect consumers and support conservation measures.
D T, Lau, P C, Shaw, J, Wang, P P, But
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Putative group I introns in the eukaryote nuclear internal transcribed spacers
Current Genetics, 2019Group I introns are mobile genetic elements that interrupt genes encoding proteins and RNAs. In the rRNA operon, introns can insert in the small subunit (SSU) and large subunit (LSU) of a wide variety of protists and various prokaryotes, but they were never found in the ITS region.
Daniele Corsaro, Danielle Venditti
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Internal Transcribed Spacer (ITS)-PCR Identification of MRSA
2007Polymerase chain reaction (PCR) analysis of the 16S-23S rRNA gene internal transcribed spacer (ITS) followed by microchip gel electrophoresis was useful for identification of staphylococci and for strain delineation of Staphylococcus aureus. In the study presented in this chapter, 74 ITS patterns were demonstrated among 1,188 isolated colonies of S ...
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Sequence Variation in the Ribosomal DNA Internal Transcribed Spacer of Tridacna crocea
Marine Biotechnology, 2000DNA-based genetic markers are needed to augment existing allozyme markers in the assessment of genetic diversity of wild giant clam populations. The dearth of polymorphic mitochondrial DNA regions amplified from known universal polymerase chain reaction (PCR) primers has led us to search other regions of the genome for viable sources of DNA ...
E T, Yu, M A, Juinio-Meñez, V D, Monje
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Fourteen internal transcribed spacers in the circular ribosomal DNA of Euglena gracilis
Journal of Molecular Biology, 1990Cytoplasmic ribosomes from Euglena gracilis contain 16 rRNA components. These include the typical 5 S, 5.8 S and 19 S rRNAs that are found in other eukaryotes as well as 13 discrete small RNAs that interact to form the equivalent of eukaryotic 25-28 S rRNA (accompanying paper).
M N, Schnare, J R, Cook, M W, Gray
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Evolutionary divergence of ribosomal internal transcribed spacer 2 in lizards
Molecular Biology, 2006Internal transcribed spacers 1 and 2 (ITS1 and ITS2) are known to play an important role in rRNA maturation, yet the mechanism of their action is still not completely understood. Comparison of the ITS1 and ITS2 nucleotide sequences for various organisms reveals conserved regions, which are potentially involved in rRNA biogenesis, and yields new ...
A. S. Voronov +3 more
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Length variation in the internal transcribed spacer of ribosomal DNA in chanterelles
Mycological Research, 1994DNA was extracted from 31 basidiomes of Cantharellus, Craterellus , and other basidiomycetes considered to have affinities to the Cantharellaceae. The internal transcribed spacer (ITS) between the 18S and the 28S genes of the nuclear ribosomal DNA was amplified using the polymerase chain reaction.
Toby Feibelman +2 more
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[Comparison of rDNA internal transcribed spacer sequences in asparagus].
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 2011Using ITS sequence of nine species to identify counterfeiting medicine and analyse phylogenetic of Asparagus.Analysing ITS sequences by amplification, cloning,sequencing and alignment.The length range of ITS sequence of nine species was from 711 to 748 bp, the percentage of G + C content was about 60%.
Li-Jun, Ou +6 more
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2012
Molecular approaches are now being developed to provide a more rapid and objective identification compared to traditional phenotypic methods. Nuclear ribosomal DNA (nrDNA) targets, especially internal transcribed spacer 1 and 2 (ITS1 and ITS2), have been widely used for molecular identification of some plants and fungi.
Yan-Lin, S +3 more
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Molecular approaches are now being developed to provide a more rapid and objective identification compared to traditional phenotypic methods. Nuclear ribosomal DNA (nrDNA) targets, especially internal transcribed spacer 1 and 2 (ITS1 and ITS2), have been widely used for molecular identification of some plants and fungi.
Yan-Lin, S +3 more
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การระบุสายพันธุ์แมลงวันด้วยสัณฐานวิทยา ไม่เพียงต้องอาศัยความเชี่ยวชาญอย่างสูงด้านอนุกรมวิธาน แต่ยังจำเป็นต้องใช้ตัวอย่างที่สมบูรณ์อีกด้วย ปัจจุบันเทคนิคการระบุสายพันธุ์โดยใช้ลำดับนิวคลีโอไทด์ถูกใช้อย่างแพร่หลาย งานวิจัยนี้ใช้ลำดับนิวคลีโอไทด์บริเวณ second internal transcribed spacer (ITS2) ของ ribosomal DNA ในการจำแนกสายพันธุ์แมลงวันที่มีความสำค ...
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