Results 81 to 90 of about 41,661 (301)

Data-Driven Models for Predicting Intrinsically Disordered Protein Polymer Physics Directly from Composition or Sequence

, 2023
The molecular-level understanding of intrinsically disordered proteins is challenging due to experimental characterization difficulties. Computational understanding of IDPs also requires fundamental advances, as the leading tools for predicting protein ...
Shiv , Rekhi, Daniel P., Tabor, Tzu-Hsuan, Chao, Jeetain, Mittal   +3 more
core   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae

Nature Communications
Disordered protein sequences can exhibit different binding modes, ranging from well-ordered folding-upon-binding to highly dynamic fuzzy binding. The primary function of the intrinsically disordered region of the antitoxin HigA2 from Vibrio cholerae is ...
San Hadži, Zala Živič, Matic Kovačič, Uroš Zavrtanik, Sarah Haesaerts, Daniel Charlier, Janez Plavec, Alexander N. Volkov, Jurij Lah, Remy Loris   +9 more
doaj   +1 more source

Long-Range Entropic Effects on Protein Intrinsically Disordered Regions

, 2019
Entropy calculations represent one of the most challenging steps in obtaining the binding free energy in proteins and their complexes, which is a grand challenge in computational biology.
Agnieszka K., Bronowska, Joao Victor de Souza Cunha (6310883), Joao Victor, de Souza Cunha, Agnieszka K. Bronowska (6320289)   +3 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Intrinsically disordered proteins and structured proteins with intrinsically disordered regions have different functional roles in the cell.

PLoS ONE, 2019
Many studies about classification and the functional annotation of intrinsically disordered proteins (IDPs) are based on either the occurrence of long disordered regions or the fraction of disordered residues in the sequence.
Antonio Deiana, Sergio Forcelloni, Alessandro Porrello, Andrea Giansanti   +3 more
doaj   +1 more source

Ensemble Docking for Intrinsically Disordered Proteins

Journal of Chemical Information and Modeling
Abstract Intrinsically disordered proteins (IDPs) are implicated in many human diseases and are increasingly being pursued as drug targets. Conventional structure-based drug design methods that rely on well-defined binding sites are however, largely unsuitable for IDPs.
Anjali Dhar, Thomas R. Sisk, Paul Robustelli   +2 more
openaire   +3 more sources

Mechanisms 
of
 change 
in 
protein 
architecture

, 2011
Proteins are the basic building blocks and functional units in all living organisms. Moreover, differences between species can frequently be explained with differences in their protein complements.

core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Elucidating ATP’s role as solubilizer of biomolecular aggregate

eLife
Proteins occurring in significantly high concentrations in cellular environments (over 100 mg/ml) and functioning in crowded cytoplasm, often face the prodigious challenges of aggregation which are the pathological hallmark of aging and are critically ...
Susmita Sarkar, Saurabh Gupta, Chiranjit Mahato, Dibyendu Das, Jagannath Mondal   +4 more
doaj   +1 more source