Results 91 to 100 of about 32,541 (302)

Tyrosinase degradation is prevented when EDEM1 lacks the intrinsically disordered region.

PLoS ONE, 2012
EDEM1 is a mannosidase-like protein that recruits misfolded glycoproteins from the calnexin/calreticulin folding cycle to downstream endoplasmic reticulum associated degradation (ERAD) pathway.
Marioara B Marin, Simona Ghenea, Laurentiu N Spiridon, Gabriela N Chiritoiu, Gabriela N Chiritoiu, Andrei-Jose Petrescu, Stefana-Maria Petrescu   +6 more
doaj   +1 more source

Intrinsically disordered protein, DNA binding with one finger transcription factor (OsDOF27) implicates thermotolerance in yeast and rice

Frontiers in Plant Science, 2022
Intrinsically disorder regions or proteins (IDRs or IDPs) constitute a large subset of the eukaryotic proteome, which challenges the protein structure–function paradigm.
Nishu Gandass, Kajal, Prafull Salvi
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

The potential of intrinsically disordered regions in vaccine development [PDF]

Expert Review of Vaccines, 2021
Mehrdad Ameri, Navid Nezafat, Sedigheh Eskandari   +2 more
openaire   +2 more sources

Enhanced synaptotagmin plasticity derived from pairing intrinsic disorder with synaptic vesicle lipids

Communicative & Integrative Biology, 2017
Synaptotagmin 1 (Syt 1) is an integral membrane protein responsible for sensing the calcium ion (Ca2+) influx in neurons that triggers synaptic vesicle exocytosis.
Michael E. Fealey, David D. Thomas, Anne Hinderliter   +2 more
doaj   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Functional analysis of the N‐terminal region of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis

FEBS Open Bio, 2022
Acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis (TTE0866) has an N‐terminal region (NTR; residues 23–135) between the signal sequence (residues 1–22) and the catalytic domain (residues 136–324), which is of unknown function ...
Kohei Sasamoto, Tomoki Himiyama, Kunihiko Moriyoshi, Takashi Ohmoto, Koichi Uegaki, Tsutomu Nakamura, Yoshiaki Nishiya   +6 more
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Disparate binding kinetics by an intrinsically disordered domain enables temporal regulation of transcriptional complex formation

, 2018
Significance Different combinations and permutations of transcription factors work together to regulate the expression of target genes. These proteins often contain high levels of intrinsically disordered regions, which are important mediators ...
Athina Manakas, Gordon McDonald, Ann H. Kwan, Neil O. Robertson, Ngaio C. Smith, Mahiar Mahjoub, Jacqueline M. Matthews   +6 more
core   +1 more source

Stop codon readthrough alters the activity of a POU/Oct transcription factor during Drosophila development

BMC Biology, 2021
Background A number of cellular processes have evolved in metazoans that increase the proteome repertoire in relation to the genome, such as alternative splicing and translation recoding.
Yunpo Zhao, Bo Gustav Lindberg, Shiva Seyedoleslami Esfahani, Xiongzhuo Tang, Stefano Piazza, Ylva Engström   +5 more
doaj   +1 more source