Results 81 to 90 of about 4,409,964 (303)

Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins

Nature Communications, 2020
The production of microparticles with complex geometries for biotechnological use historically requires sophisticated fabrication techniques. Here, the authors create complex particle geometries by exploiting the metastable region of the phase diagram of
Stefan Roberts, Vincent Miao, Simone Costa, Joseph Simon, Garrett Kelly, Tejank Shah, Stefan Zauscher, Ashutosh Chilkoti   +7 more
doaj   +1 more source

Smaller is better: nanobodies meet NMR

FEBS Letters, EarlyView.
Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley   +1 more source

Tyrosinase degradation is prevented when EDEM1 lacks the intrinsically disordered region.

PLoS ONE, 2012
EDEM1 is a mannosidase-like protein that recruits misfolded glycoproteins from the calnexin/calreticulin folding cycle to downstream endoplasmic reticulum associated degradation (ERAD) pathway.
Marioara B Marin, Simona Ghenea, Laurentiu N Spiridon, Gabriela N Chiritoiu, Gabriela N Chiritoiu, Andrei-Jose Petrescu, Stefana-Maria Petrescu   +6 more
doaj   +1 more source

Functional analysis of the N‐terminal region of acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis

FEBS Open Bio, 2022
Acetylxylan esterase from Caldanaerobacter subterraneus subsp. tengcongensis (TTE0866) has an N‐terminal region (NTR; residues 23–135) between the signal sequence (residues 1–22) and the catalytic domain (residues 136–324), which is of unknown function ...
Kohei Sasamoto, Tomoki Himiyama, Kunihiko Moriyoshi, Takashi Ohmoto, Koichi Uegaki, Tsutomu Nakamura, Yoshiaki Nishiya   +6 more
doaj   +1 more source

Soft motions encoded by the fold and mediated by conserved hinge sites drive V‐ATPase allosteric machinery and autoinhibition

FEBS Letters, EarlyView.
Vacuolar H+‐ATPase (V‐ATPase) is a multisubunit rotary proton pump that acidifies intracellular compartments. Using elastic network models, we identified conserved hinge residues that govern its large‐scale functional motions. We show that intrinsically encoded ‘soft modes’ drive critical functional transitions, including rotary catalysis and ...
Diksha Parwana, Xiaowei Bogetti, Stuti Sharma, Ivet Bahar   +3 more
wiley   +1 more source

The potential of intrinsically disordered regions in vaccine development [PDF]

Expert Review of Vaccines, 2021
Mehrdad Ameri, Navid Nezafat, Sedigheh Eskandari   +2 more
openaire   +2 more sources

Stop codon readthrough alters the activity of a POU/Oct transcription factor during Drosophila development

BMC Biology, 2021
Background A number of cellular processes have evolved in metazoans that increase the proteome repertoire in relation to the genome, such as alternative splicing and translation recoding.
Yunpo Zhao, Bo Gustav Lindberg, Shiva Seyedoleslami Esfahani, Xiongzhuo Tang, Stefano Piazza, Ylva Engström   +5 more
doaj   +1 more source

Inherent structural disorder and dimerisation of murine norovirus NS1-2 protein [PDF]

, 2012
Human noroviruses are highly infectious viruses that cause the majority of acute, non-bacterial epidemic gastroenteritis cases worldwide. The first open reading frame of the norovirus RNA genome encodes for a polyprotein that is cleaved by the viral ...
Krause Kurt L., Luckner, Sylvia R., Krause, Kurt L., Luckner Sylvia R., Lambden, Paul R., Sylvia R Luckner, Kurt L Krause, Clarke, Ian N., Paul R Lambden, Baker, Estelle S., Ian N Clarke, Baker Estelle S., Estelle S Baker, Ward Vernon K., Lambden Paul R., Ward, Vernon K., Clarke Ian N., Vernon K Ward   +17 more
core   +1 more source

Unlocking the potential of cell‐permeable nanobodies for intracellular applications

FEBS Letters, EarlyView.
This review outlines current strategies for delivery of nanobodies into cells and highlights recent examples of functional applications. By addressing and manipulating intracellular targets, these molecules hold great potential for treating cancer, respiratory diseases and neurological disorders, making this a vibrant area of research in the biomedical
Mariona Gené‐Verdés, Luise Franz, Christian P.R. Hackenberger   +2 more
wiley   +1 more source

Decoding intrinsically disordered regions in biomolecular condensates

Fundamental Research
Biomolecular condensates comprise a diverse array of molecular entities, with intrinsically disordered regions (IDRs) receiving mounting attention due to their pivotal roles. In recent years, significant progress has been made in understanding the linear and conformational molecular grammar of IDRs in biomolecular condensates. This review will focus on
Minglei Shi, Zhaoxu Wu, Yi Zhang, Tingting Li   +3 more
openaire   +2 more sources