Results 71 to 80 of about 4,746 (287)

Why do eukaryotic proteins contain more intrinsically disordered regions?

PLoS Computational Biology, 2019
Intrinsic disorder is more abundant in eukaryotic than prokaryotic proteins. Methods predicting intrinsic disorder are based on the amino acid sequence of a protein. Therefore, there must exist an underlying difference in the sequences between eukaryotic
Walter Basile, Marco Salvatore, Claudio Bassot, Arne Elofsson   +3 more
doaj   +1 more source

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

Nature Communications, 2021
The SARS-CoV-2 nucleocapsid (N) protein binds the viral RNA genome and contains two ordered domains flanked by three intrinsically-disordered regions. Here, the authors show that RNA binding induces liquid-liquid phase separation of N, which is driven by
Shan Lu, Qiaozhen Ye, Digvijay Singh, Yong Cao, Jolene K. Diedrich, John R. Yates, Elizabeth Villa, Don W. Cleveland, Kevin D. Corbett   +8 more
doaj   +1 more source

Discovery of short linear motif-mediated interactions through phage display of intrinsically disordered regions of the human proteome.

, 2016
The intrinsically disordered regions of eukaryotic proteomes are enriched in short linear motifs (SLiMs), which are of crucial relevance for cellular signaling and protein regulation; many mediate interactions by providing binding sites for peptide ...
Yadav, V.
core   +1 more source

The potential of intrinsically disordered regions in vaccine development [PDF]

Expert Review of Vaccines, 2021
Mehrdad Ameri, Navid Nezafat, Sedigheh Eskandari   +2 more
openaire   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Long-Range Entropic Effects on Protein Intrinsically Disordered Regions

, 2019
Entropy calculations represent one of the most challenging steps in obtaining the binding free energy in proteins and their complexes, which is a grand challenge in computational biology.
Agnieszka K., Bronowska, Joao Victor de Souza Cunha (6310883), Joao Victor, de Souza Cunha, Agnieszka K. Bronowska (6320289)   +3 more
core   +1 more source

Machine-learning analysis of intrinsically disordered proteins identifies key factors that contribute to neurodegeneration-related aggregation

Frontiers in Aging Neuroscience, 2022
Protein structure is determined by the amino acid sequence and a variety of post-translational modifications, and provides the basis for physiological properties.
Akshatha Ganne, Meenakshisundaram Balasubramaniam, Srinivas Ayyadevara, Srinivas Ayyadevara, Srinivas Ayyadevara, Robert J. Shmookler Reis, Robert J. Shmookler Reis, Robert J. Shmookler Reis   +7 more
doaj   +1 more source

Intrinsically Disordered Proteins: An Overview

, 2022
Many proteins and protein segments cannot attain a single stable three-dimensional structure under physiological conditions; instead, they adopt multiple interconverting conformational states.
Trivedi, Rakesh, Nagarajaram, Hampapathalu Adimurthy   +1 more
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Expose flexible conformations for intrinsically disordered protein

Current Research in Structural Biology
The folding conformation of native protein has flexibility in different degrees, which may bring difficulty in presenting the structures, and also it causes complexity in understanding the relationship between structure and functions.
Jiaan Yang, Wenxin Ji, Wen Xiang Cheng, Gang Wu, Si Tong Sheng, Peng Zhang, Jun Lin, Xiaojia Chen, Qiong Shi   +8 more
doaj   +1 more source