Results 71 to 80 of about 165,293 (290)

Rapid ion-exchange chromatography for preparative separation of proteins IV. Application to bovine carbonic anhydrase III from skeletal muscle [PDF]

, 1991
Bovine muscle carbonic anhydrase III was purified to homogeneity by the strategy of rapid ion-exchange chromatography. The ionic exchanger used was CM-cellulose, and this is the first application of this technique on a cation exchanger.
Kristina Borén, Borén, Kristina,, Uno Carlsson, Nils Bergenhem, Bergenhem, Nils,, Carlsson, Uno,, Marie Larsson, Larsson, Marie,   +7 more
core   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

Analysis of metabolic profiles of bile acids in urine using a lipophilic anion exchanger and computerized gas-liquid chromatorgaphy-mass spectrometry

Journal of Lipid Research, 1977
A method is described for quantitative analysis of bile acids in urine. Urine is acidified and bile acids are extracted on an Amberlite XAD-2 column.
B Almé, A Bremmelgaard, J Sjövall, P Thomassen   +3 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

The fabrication and modification of capillary polymer monoliths for the separation of small ions [PDF]

, 2012
The fabrication and modification of polymer monoliths, in capillary formats, for the separation of small ions is presented. The separation of small ions using polymer monoliths has limitations and this work aims to investigate increasing the ion exchange
Moyna, Aine
core  

Design of two-column batch-to-batch recirculation to enhance performance in ion-exchange chromatography [PDF]

, 2018
Preparative liquid chromatography is a separation technique widely used in the manufacturing of fine chemicals and pharmaceuticals. A major drawback of traditional single-column batch chromatography step is the trade-off between product purity and ...
Nilsson, Bernt,, Lund University., Andersson, Niklas,, Persson, Oliver,   +3 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

ION EXCHANGE CHROMATOGRAPHY WITH CONDUCTIVITY DETECTION COMPARED TO SPECTROPHOTOMETRY TO ASSAY PHOSPHATE IN MILK AND MILK PRODUCTS

Jurnal Teknologi dan Industri Pangan, 2015
Phosphorus is a critical dietary mineral that is involved as phosphate in bone, tooth, nucleic acids or phospholipids and is mainly supplied through consumption of dairy products.
Tia Amina Setiawati, Fanny Guyomarc’h, Eric Beaucher, Frederic Gaucheron   +3 more
doaj   +1 more source

Production and Purification of a Polyclonal Antibody Against Purified Mouse IgG2b in Rabbits Towards Designing Mouse Monoclonal Isotyping Kits [PDF]

Advanced Pharmaceutical Bulletin, 2015
Purpose: Mouse IgG subclasses containing IgG1, IgG2a, IgG2b and IgG3 have been defined and described both physiochemically and immunologically. Methods: Sepharose beads conjugated with protein A affinity chromatography was used for purification of mouse
Sadeq Eivazi, Jafar Majidi, Leili Aghebati Maleki, Jalal Abdolalizadeh, Mehdi Yousefi, Majid Ahmadi, Somayeh Dadashi, Zahra Moradi, Elmira Zolali   +8 more
doaj   +1 more source

A comparative study of ion exchange vs. ion pair chromatography for preparative separation of oligonucleotides [PDF]

Oligonucleotides are commonly purified using either ion exchange chromatography (IEX) or ion-pair reversedphase liquid chromatography (IP-RPLC). This study compares the purification of a crude 20-mer oligonucleotide (ON) using both methods under ...
Thunberg, Linda, Fornstedt, Torgny, Unoson, Cecilia,, Samuelsson, Jörgen, Stavenhagen, Kathrin,, Borland, Kayla,, Unoson, Cecilia, Jora, Manasses, Samuelsson, Jörgen,, Stavenhagen, Kathrin, Stalberg, Olof,, Leek, Tomas, Jora, Manasses,, Borland, Kayla, Leek, Tomas,, Lesko, Marek, Stalberg, Olof, Enmark, Martin,, Lesko, Marek,, Thunberg, Linda,, Fornstedt, Torgny,, Enmark, Martin   +21 more
core   +1 more source