Results 91 to 100 of about 165,016 (280)

ISOLATION AND PURIFICATION OF GLUCOSYLTRANSFERASE FROM MUTANS STREPTOCOCCUS SOBRINUS (SEROTYPE G) LOCAL ISOLATE

The Iraqi Journal of Medical Sciences, 2016
Background: Glucosyltransferase is an extracellular enzyme produced by mutans streptococci responsible for polymerizing the glucose moiety of sucrose to form glucan.
Nada H. Al-Mudallal, Essam F. Al-Jumaily, Nidhal Abdul Muhymen, Abd Al- Wahid Al-Shaibany   +3 more
doaj   +6 more sources

Purification of bovine pancreatic glucagon as a by-product of insulin production

Brazilian Journal of Medical and Biological Research, 1997
A process for purifying bovine pancreatic glucagon as a by-product of insulin production is described. The glucagon-containing supernatant from the alkaline crystallization of insulin was precipitated using ammonium sulfate and isoelectric precipitation.
A.S.R. Andrade, L. Vilela, H. Tunes
doaj   +1 more source

Enhancing Selectivity of Protein Biopharmaceuticals in Ion Exchange Chromatography through Addition of Organic Modifiers. [PDF]

Int J Mol Sci, 2023
Duivelshof BL, Bouvarel T, Pirner S, Larraillet V, Knaupp A, Koll H, D'Atri V, Guillarme D.   +7 more
europepmc   +1 more source

Functional Analysis of Ligand‐Gated Chloride Channels in a Cnidarian Sheds Light on the Evolution of Inhibitory Signaling

Advanced Science, EarlyView.
We uncover a large variety of putative inhibitory ligand‐gated ion channels (LGICs) in the phylum Cnidaria, the sister group to all bilaterian animals. Phylogenetic analysis suggests a complex evolutionary history of inhibitory LGICs with diverse neurotransmitter ligands.
Abhilasha Ojha, Linda Kloss, Juan D. Montenegro, Simone Albani, Audrey Ortega‐Ramírez, Mihaela Raycheva, Sylvia Joussen, Sabrina Kaul, Michèle Bachmann, Lisa Huf, Günther Schmalzing, Alison G. Cole, Ulrich Technau, Stefan Gründer   +13 more
wiley   +1 more source

A comparative study of ion exchange vs. ion pair chromatography for preparative separation of oligonucleotides

Oligonucleotides are commonly purified using either ion exchange chromatography (IEX) or ion-pair reversedphase liquid chromatography (IP-RPLC). This study compares the purification of a crude 20-mer oligonucleotide (ON) using both methods under ...
Thunberg, Linda, Fornstedt, Torgny, Unoson, Cecilia,, Samuelsson, Jörgen, Stavenhagen, Kathrin,, Borland, Kayla,, Unoson, Cecilia, Jora, Manasses, Samuelsson, Jörgen,, Stavenhagen, Kathrin, Stalberg, Olof,, Leek, Tomas, Jora, Manasses,, Borland, Kayla, Leek, Tomas,, Lesko, Marek, Stalberg, Olof, Enmark, Martin,, Lesko, Marek,, Thunberg, Linda,, Fornstedt, Torgny,, Enmark, Martin   +21 more
core   +1 more source

Microscopic insight into role of protein flexibility during ion exchange chromatography by nuclear magnetic resonance and quartz crystal microbalance approaches

, 2016
Driven by the prevalent use of ion exchange chromatography (IEC) for polishing therapeutic proteins, many rules have been formulated to summarize the different dependencies between chromatographic data and various operational parameters of interest based
Zhao, Lan, Ma, GH, Ma, Guanghui, Su, Zhiguo, Hao, Dongxia, Huang, Yongdong, Ge, Jia   +6 more
core   +1 more source

Corrosion behavior of aluminum in ion exchange resin leachates from cooling systems in high-voltage direct current transmission

International Journal of Electrochemical Science
The corrosion behavior of aluminum radiators in immersion solutions containing anion exchange resin (IRA), cation exchange resin (IRC), and mixed anion-cation exchange resin (IRM) from converter valve cooling systems in high-voltage direct current (HVDC)
Feng Wang, Xue Han, Liangshou Hao, Han Xu, Chaoyu Lei, Hong Xu, Jiayang Li, Yao Cheng, Shengping Wang   +8 more
doaj   +1 more source

Purification of Erythropoietin by Ion-Exchange Chromatography.

Experimental Biology and Medicine, 1958
Abstract : A method is given for the purification of erythropoietin from the filtrate of acidified, boiled plasma prepared from phenylhydrazine anemic rabbits utilizing, DEAE-cellulose ion-exchange columns. The active erythropoietic factor thus prepared has been partially characterized and shown to be an acidic glycoprotein of low molecular weight.
W A, RAMBACH, J A, COPPER, H L, ALT
openaire   +2 more sources

Heme‐NO Dilates Arteries via Mobilization of NO Moieties From an Intracellular NO Store Within Vascular Smooth Muscle Cells

Advanced Science, EarlyView.
Nitrosyl heme emerges as an extracellular nitrodilator that dilates arteries without crossing the cell membrane. Instead, heme‐NO mobilizes NO moieties from a preformed intracellular NO store within vascular smooth muscle, providing both functional and chemical evidence for the NANOS model, revealing a previously unrecognized mechanism of arterial ...
Taiming Liu, Meijuan Zhang, Lingchao Zhu, Haiyan Ke, Amancio de Souza, Qian Li, Daniel Castella, Nicolai Lehnert, Lubo Zhang, Arlin B. Blood   +9 more
wiley   +1 more source

ZDHHC9‐Mediated Palmitoylation of ACSL4 Drives Ferroptosis in Diabetes Mellitus–Induced Erectile Dysfunction

Advanced Science, EarlyView.
ABSTRACT Diabetes mellitus‐induced erectile dysfunction (DMED) is a highly prevalent complication among diabetic patients; however, its underlying pathogenic mechanisms remain incompletely understood. Metabolic disorder is a hallmark of diabetes, yet its precise contribution to DMED progression is not well defined.
Wanyang Guo, Ming Xiao, Mengjun Huang, Dongzi Peng, Ruijiang Zeng, Ruilin Liu, Yuanqiao Zhao, Zhihan Ouyang, Yulong Hong, Zexian Ding, Zhuo Xing, Hao Su, Jinxiang Wang, Wenjun Mao, Xin Jin   +14 more
wiley   +1 more source