Results 191 to 200 of about 3,213,552 (278)

Purification of Disassembled Capsid Protein by Hydroxyapatite Chromatography Enhances Virus‐Like Particle Recovery

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Virus‐like particles (VLPs) are a promising modality with extensive applications in prophylactic and therapeutic vaccine design. Human Papillomavirus (HPV) vaccines are one such example where VLP vaccine applications have led to the successful reduction in HPV‐associated diseases, such as cervical cancer.
Nicholas Samuelson, Nathaniel Parker, Shiyi Wang, Sara Daley, Aaron Cote, Michael A. Winters   +5 more
wiley   +1 more source

Ion-exchange resin

NIPPON SHOKUHIN KOGYO GAKKAISHI, 1984
openaire   +4 more sources

Nuclease‐Free Scalable Purification of Physalis Mottle Virus‐Like Particles Expressed in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Plant virus‐like particles (VLPs) are promising nanotechnology platforms, but their translation requires purification strategies suitable for large‐scale production. Here, we developed a nuclease‐free downstream process for Physalis mottle virus (PhMV) VLPs expressed in Escherichia coli, combining ammonium sulfate precipitation, low‐pH ...
Sean K. Hsu, Patrick Opdensteinen, Tariq Hussain, Nicole F. Steinmetz   +3 more
wiley   +1 more source

Feasibility Assessment of a Single‐pH Connected Polishing Train for Monoclonal Antibody Purification Using Mildly Acidic Protein A Elution and an Acidic‐Window Anion Exchange Membrane

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody downstream processing often requires multiple pH adjustments between Protein A capture and polishing steps. These adjustments increase process complexity and make direct unit‐operation connection more difficult. We evaluated a connected post‐capture polishing block operated at approximately pH 5.0 using mild‐pH Protein A ...
Takaki Aiso, Hiroshi Muto, Kyota Suzuki, Yumiko Masuda, Hirofumi Kakihara, Masashi Sonoyama   +5 more
wiley   +1 more source

Effects of Addition of Lanthanum and Zinc Oxides on the Biological Properties of TiO₂-SiO₂-P₂O₅/CaO on Ion Exchange Resin for Bone Implantation. [PDF]

ACS Omega
Lyutova ES, Tkachuk VA, Zakharkiva AM, Borilo LP, Buzaev AA, Chen YW.   +5 more
europepmc   +1 more source

Serotype‐Tailored Midstream and Downstream Operations to Advance the Manufacturing of Adeno‐Associated Viral Vectors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viruses (AAVs) are the leading vectors for in vivo gene therapy, fueling a growing demand for scalable and robust biomanufacturing processes. Yet, the impact of cell lysis conditions on the performance of midstream and downstream unit operations across serotypes remains poorly characterized.
Arianna Minzoni, Shriarjun Shastry, Eduardo Barbieri, Morgan R. Hurst, Kelvin Idanwekhai, Yuxuan Wu, Katherine Ernst, Wai‐Keen Chung, Christopher Bombeck, Saad A. Khan, Sushmita Koley, Gabriella Kiss, Oliver Rammo, Alexander Tropsha, Cristiana Boi, Michael A. Daniele, Steven Cramer, Barry Byrne, Stephen Kaminsky, Angie Snell Bennett, Stefano Menegatti   +20 more
wiley   +1 more source

Batch‐to‐Batch Optimization of pH Shifts in CHO Bioreactor Operations

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Culture pH is an important operating parameter in CHO cell cultures producing mAb since it directly affects growth and specific productivity. Mathematical models can be used to optimize the magnitude and timing of pH shifts provided that they are robust to model errors.
Ali Ghodba, Zahra Negahban, Anne Richelle, Chris McCready, Valerie Ward, Luis Ricardez‐Sandoval, Hector Budman   +6 more
wiley   +1 more source

A Microfluidic TFF Device for Biopharmaceutical Process Development: Scale‐Down Verification of Ultrafiltration and Diafiltration Modes for Monoclonal Antibodies

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Tangential flow filtration (TFF) is an essential operation in downstream bioprocessing, yet conventional scale‐down models require large material volumes and often fail to capture scale‐relevant hydrodynamics. A microfluidic TFF (µTFF) platform was developed that preserves wall shear rate and transmembrane pressure, enabling low‐volume ...
Nusrat Jahan, Nicolas Szita, Spyridon Konstantinidis, Marco P. C. Marques   +3 more
wiley   +1 more source

Design principles for vitrimers: Linking dynamic covalent chemistry, network architecture, and engineering performance

Bulletin of the Korean Chemical Society, EarlyView.
This review examines vitrimer performance from a broader perspective, considering not only dynamic covalent chemistry but also molecular structure, network architecture, and rheological behavior. These factors are discussed in relation to service and processing windows, reprocessing, repair, and engineering applications, providing a multiscale ...
Kyosun Ku, Hyeonuk Yeo
wiley   +1 more source

Structural compatibility between β‐lactams and substrate binding pockets of IMP‐1 and NDM‐1 determines the substrate spectrum

Bulletin of the Korean Chemical Society, EarlyView.
Chemical structures of three β‐lactam classes and the substrate‐binding pockets (SBPs) of B1 metallo‐β‐lactamases (MBLs) were compared. Structural compatibility between β‐lactams and MBL SBPs correlates with enzymatic activity, providing insights into the substrate spectrum of MBLs and β‐lactam resistance.
Kiwoong Kwak, Jiwon Yun, Donghyun Lee, Byung‐Jin Lee, Juyeon Lim, Jung Hun Lee, Sang Hee Lee, Soon Young Shin, Lin‐Woo Kang   +8 more
wiley   +1 more source