Results 201 to 210 of about 3,213,552 (278)

Kinetic Study on Esterification of Acetic Acid with Isopropyl Alcohol Catalyzed by Ion Exchange Resin. [PDF]

ACS Omega, 2019
Liu Y, Liu J, Yan H, Zhou Z, Zhou A.
europepmc   +1 more source

Mitochondria‐endoplasmic reticulum organelle glue as maturation promoter for origination/dimensional dual‐cross cardiomyocytes

BMEMat, EarlyView.
Mitochondria‐endoplasmic reticulum contact sites (MERCS) are areas where the mitochondria and endoplasmic reticulum closely interact. In this study, we utilize synthetic organelle glues to artificially engineer MERCS for regulating cardiomyocyte development, through which the immature and chemo‐plasticity issues of undifferentiated cells are addressed.
Wei Tang, Hao Sun, Chenqi Duan, Jiajia Chang, Yuxing Wang, Ming Zhao, Huiyue Zhao, Shan Lu, Yang Yang, Da Huo   +9 more
wiley   +1 more source

High‐throughput development and scale‐up of recombinant adeno‐associated virus 5 anion‐exchange chromatography for high‐purity capsid separation

Biotechnology Progress, EarlyView.
High‐throughput anion‐exchange chromatography process development enables scalable purification of high‐purity recombinant adeno‐associated virus capsids. Abstract Recombinant adeno‐associated virus (rAAV) production commonly yields a high proportion of empty and partially filled capsids, necessitating downstream purification to enrich full capsids ...
Kevin Vera, Ivan Gajic, Praveen Kusumanchi, Angela Andaluz, Gabrielle Dela Pena, Clifford A. Froelich, Elizabeth Higgins, Jennifer Johnston, Seyed Pouria Motevalian   +8 more
wiley   +1 more source

Multifaceted Ion Exchange Resin-Supported Hydrogen Fluoride: A Path to Flow Hydrofluorination. [PDF]

Green Chem, 2019
Lu Z, Bajwa BS, Okoromoba OE, Hammond GB, Xu B.   +4 more
europepmc   +1 more source

DNA concentration in harvest affects retrovirus clearance capabilities of protein A chromatography

Biotechnology Progress, EarlyView.
Abstract Protein A chromatography is a key unit operation in biopharmaceutical purification, widely used to capture monoclonal antibodies (mAbs) from harvest cell culture fluid (HCCF). In addition to its primary function, protein A chromatography is also frequently tested for its ability to remove viruses. Although protein A specifically binds mAbs and
Lukas Döring, Sven Schubert, Nora Roos, Johannes Winderl, Annika Müller, Anna Ratermann, Matthias W. Kron, Jürgen Hubbuch   +7 more
wiley   +1 more source

Epoxidation Kinetics of High-Linolenic Triglyceride Catalyzed by Solid Acidic-Ion Exchange Resin. [PDF]

Sci Rep, 2019
Kousaalya AB, Beyene SD, Ayalew B, Pilla S.   +3 more
europepmc   +1 more source

Development and application of a mechanistic model for protein A resolution of a bispecific antibody for process characterization and optimization

Biotechnology Progress, EarlyView.
Abstract Regeneron's bispecific technology, based on a fully human IgG antibody construct, consists of two distinct heavy chains and a common light chain co‐expressed in a single cell. The co‐expression of the two distinct heavy chains, one of which contains a dipeptide substitution that ablates Protein A binding, leads to the formation of three ...
Samantha Wadsworth, James Reilly, John Mattila, Christopher Cowan, Hanne Bak   +4 more
wiley   +1 more source

Rapid assessment of heavy metal pollution using ion-exchange resin sachets and micro-XRF core-scanning. [PDF]

Sci Rep, 2019
Huang JS, Lin SC, Löwemark L, Liou SYH, Chang Q, Chang TK, Wei KY, Croudace IW.   +7 more
europepmc   +1 more source

Flocculation using selected cationic polymers reduces high‐risk host cell proteins in monoclonal antibody processes already during primary recovery

Biotechnology Progress, EarlyView.
Flocculation using cationic polymers leads to the creation of large floc complexes. Negative charged species like cells, cell debris, and negatively charged host cell impurities like host cell DNA and host cell proteins are trapped within the complex while the positively charged monoclonal antibody stays in solution.
Anna‐Carina Frank, Daniel Komuczki, Gregor Stitz, Timon Kalchmayr, Clemens Grünwald‐Gruber, Clara Hofmann, Natalie Deiringer, Christine Rösch, Peter Satzer, Rainer Hahn   +9 more
wiley   +1 more source