Results 31 to 40 of about 353,875 (266)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Analysis of the ion mobility spectra of chloroacetophenone, tris(2-chloroethyl)amine, and methanethiol

Тонкие химические технологии
Objectives. To determine the ion mobilities of chloroacetophenone, tris(2-chloroethyl)amine, and methanethiol; the structure of ions corresponding to characteristic signals; the detection limits of chloroacetophenone, tris(2-chloroethyl)amine, and ...
D. A. Aleksandrova, T. B. Melamed, E. P. Baberkina, E. S. Osinova, L. A. Luzenina, A. A. Kaplin, R. V. Yakushin, A. E. Kovalenko, G. V. Tsaplin, Yu. B. Sinkevich, A. A. Fenin, J. R. Shaltaeva, V. V. Belyakov, A. O. Shablya, A. G. Sazonov   +14 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Mathematical Models for Acoustic Spectra Simulation

Communications, 2010
The experimentally obtained acoustic spectra of some investigated materials; particularly the ion conductive glasses and MOS (metal-oxide-semiconductor) structures are analyzed using suitable theoretical models and mathematical procedure to fit the ...
Peter Hockicko, Peter Bury, Peter Sidor, Stanislav Jurecka, Igor Jamnicky   +4 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Modeling Ion Acceleration and Transport in Corotating Interaction Regions: The Mass-to-charge Ratio Dependence of the Particle Spectrum

The Astrophysical Journal Letters
We investigate the role of perpendicular diffusion in shaping the energetic ion spectrum in corotating interaction regions (CIRs), focusing on its mass-to-charge ( A / Q ) ratio dependence.
Zheyi Ding, Gang Li, Nicolas Wijsen, Stefaan Poedts, Shuo Yao   +4 more
doaj   +1 more source

Time-of-flight secondary ion mass spectrometry fragment regularity in gallium-doped zinc oxide thin films

Scientific Reports, 2021
Time-of-flight secondary ion mass spectrometry fragment analysis remains a challenging task. The fragment appearance regularity (FAR) rule is particularly useful for two-element compounds such as ZnO.
K. G. Saw, S. R. Esa
doaj   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source