Results 261 to 270 of about 1,162,423 (312)

Isolation of Canine Neutrophil Plasma Membranes

Journal of Leukocyte Biology, 1985
Abstract This paper describes the isolation of plasma membrane vesicles formed by nitrogen cavitation of canine neutrophils. Plasma membranes from disrupted cells were separated from other membranes and organelles by Percoll-density gradient centrifugation.
R T, O'Donnell, B R, Andersen
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Physical chemistry of isolated bacterial membranes

Biochimica et Biophysica Acta (BBA) - General Subjects, 1965
Abstract The membranes (plasma-mesosome system) isolated from Micrococcus lysodeekticus and Sarcina lutea have been dissociated with the non-ionic detergent, “Nonidet P.40” (active constituent of the polyoxyethylated alkyl phenol type), to give major components in the ultracentrifuge having sedimentation coefficients (sin20,w) of 3.1 and 3.3 ...
M R, Salton, A, Netschey
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Rotavirus interaction with isolated membrane vesicles

Journal of Virology, 1994
To gain information about the mechanism of epithelial cell infection by rotavirus, we studied the interaction of bovine rotavirus, RF strain, with isolated membrane vesicles from apical membrane of pig enterocytes. Vesicles were charged with high (quenching) concentrations of either carboxyfluorescein or calcein, and the rate of fluorophore release ...
Ruiz, M.C., Alonso-Torre, S.R., Charpilienne, Annie, Vasseur, M., Michelangeli, F., Cohen, J., Alvarado, F.   +6 more
openaire   +3 more sources

Isolation of mitochondria-associated ER membranes

2020
Organelles within cells are interconnected by physical associations or contact sites. In the last decade, many reports have shown that these interactions are functional domains that maintain cellular homeostasis. One of the best studied interactions is between endoplasmic reticulum (ER) and mitochondria via mitochondria-associated membranes or MAMs ...
Jorge, Montesinos, Estela, Area-Gomez
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Plasma membranes from isolated liver cells

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1972
Abstract Plasma membranes have been prepared from isolated rat or guinea pig liver cells by a method established for whole liver. However, plasma membranes of liver cells isolated by disruption of the tissue with collagenase and hyaluronidase are in some detail dissimilar to those obtained from whole liver.
A, Solyom, C J, Lauter, E G, Trams
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Isolation of Intestinal Brush‐Border Membranes

Current Protocols in Cell Biology, 2006
AbstractThis unit provides protocols for isolating intestinal brush‐border membranes from rat, pig, and cow. These membranes can be used for immunoblotting or other analytical techniques. They will also spontaneously form closed vesicles which allow for flux assays to be performed using rapid filtration techniques. Overall the isolation procedures take
Chris I, Cheeseman, Debbie, O'Neill
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Membrane biogenesis on isolated protoplasma surface

Proceedings, annual meeting, Electron Microscopy Society of America, 1978
A number of papers show the formation of a new membrane on the surface of protoplasm run out after cutting of Characeae alga cells in a medium containing bivalent cations.The drops of isolated protoplasm were prepared by cutting of Nitella cells in a special decompression chamber under the conditions of turgor pressure compensation.
Kuzevanov, Victor, Salyaev, R.K., Chernyshov, V.I.   +2 more
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Calcification of Membranes Isolated from

Journal of Dental Research, 1976
Lytic cultures of Bacterionema matruchotii were found to release vesicular membranes into the medium which could be obtained virtually free of other cell structures by differential centrifugation. Suspension of the membrane fraction in a metastable calcium phosphate solution resulted in the formation of both amorphous mineral and hydroxyapatite ...
J J, Vogel, W N, Smith
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Isolation of plasma membrane–associated membranes from rat liver

Nature Protocols, 2014
Dynamic interplay between intracellular organelles requires a particular functional apposition of membrane structures. The organelles involved come into close contact, but do not fuse, thereby giving rise to notable microdomains; these microdomains allow rapid communication between the organelles.
Suski JM, Lebiedzinska M, Wojtala A, Duszynski J, GIORGI, Carlotta, PINTON, Paolo, Wieckowski MR   +6 more
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Isolating Extraembryonic Membranes

Cold Spring Harbor Protocols, 2006
Andras, Nagy, Marina, Gertsenstein, Kristina, Vintersten, Richard, Behringer   +3 more
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