Results 111 to 120 of about 1,454,719 (345)

NSP7 Molecular Degrader Attenuates Coronaviral Infection Through the β‐TrCP1/FBXO5 Axis

Advanced Science, EarlyView.
Host E3 ligase FBXO5 as a key regulator that promotes K48‐linked ubiquitination and proteasomal degradation of SARS‐CoV‐2 NSP7, thereby suppressing viral replication. NSP7 ubiquitination is co‐regulated by β‐TrCP1 and TAF1. Small molecule BC24877 disrupts β‐TrCP1‐mediated degradation of FBXO5, stabilizing FBXO5 and enhancing NSP7 clearance ...
Yao Tong, Travis B. Lear, Ferhan Tuncer, John J. Villandre, Áine N. Boudreau, Bo Lin, Irene Alfaras, Jason R. Kennerdell, Daniel P. Camarco, Mads B. Larsen, Yun Hua, Yanwen Chen, Meigin E. Chandler, Ricardo Pineda, Simon M. Barratt‐Boyes, John W. Evankovich, Toren Finkel, Yuan Liu, Bill B. Chen   +18 more
wiley   +1 more source

Native-PAGE analysis of protein aggregation upon viral infection in mouse macrophages

STAR Protocols, 2022
Summary: Upon viral infection, several proteins in the innate signaling pathway form aggregates, which in turn promote the activation of innate antiviral immune response.
Zhenzhen Yan, Hansen Liu, Chengjiang Gao
doaj  

Isolation of Pathogens Causing Sepsis, Pus and Infected Wounds from Critical Care Unit: A Retrospective Study

, 2015
Postsurgical wound infections are one of the important cause of morbidity and mortality worldwide. Therefore A retrospective study was conducted to evaluate microorganisms responsible for wound infections and their patterns of antibiotic sensitivity and ...
M. Ayub, Huma Rizwan, S. Siddique, Ushna Maryam   +3 more
semanticscholar   +1 more source

Neutrophil Macrophage Crosstalk via Extracellular Vesicles Drives Reverse Migration in a Fully Human Model of Wound Healing

Advanced Science, EarlyView.
A novel microphysiological system that can mimic sterile or non‐sterile injury demonstrates that macrophage‐derived extracellular vesicles modulate neutrophil migratory responses to these different wound states. The model showed that resolution of inflammation through neutrophil reverse migration is driven by IL‐8 derived from extracellular vesicles ...
Kehinde Adebayo Babatunde, Oluwadamilola Fatimat Babatunde, Adeel Ahmed, Wilmara Salgado‐Pabon, David J Beebe, Sheena C. Kerr   +5 more
wiley   +1 more source

In vitro infection of primary chicken lymphocytes with Marek’s disease virus

STAR Protocols, 2023
Summary: Marek’s disease virus (MDV) is a highly oncogenic alphaherpesvirus that infects immune cells and causes a deadly lymphoproliferative disease in chickens.
Luca D. Bertzbach, Marina Kohn, Yu You, Lisa Kossak, Mohammad A. Sabsabi, Ahmed Kheimar, Sonja Härtle, Benedikt B. Kaufer   +7 more
doaj  

Isolation rate, T-serotyping and susceptibility to antibiotics of Group A Streptococcus from pediatric infections in Athens* *This work was presented in part at the 7th European Congress of Clinical Microbiology and Infectious Diseases (Vienna, Austria) March 26–30, 1995 (Abstract 1349). [PDF]

, 2000
Maria Kanellopoulou, A. Makri, H. Damaskopoulou, H. Malamou‐Lada   +3 more
openalex   +1 more source

tRF‐5004b Enriched Secretory Autophagosomes Induce Endothelial Cell Activation to Drive Acute Respiratory Distress Syndrome

Advanced Science, EarlyView.
Acute respiratory distress syndrome (ARDS) is a severe lung condition with limited treatments, often triggered by overactive endothelial cells (ECs). It is identified that macrophage‐derived secretory autophagosomes (MSAPs) exacerbate lung damage by activating EC function.
Xing‐xing Zhu, Han Li, Shuang‐feng Zi, Ying Tang, Na Liu, Ke‐xin Li, Shi‐ke Geng, Hui‐qing Lu, Zhi‐kang Xie, Xin‐yi Xu, Yi‐ting Wei, Li‐xin Wang, Tao Liu, Jie Chao, Yi Yang, Hai‐bo Qiu, Wei Huang, Ling Liu   +17 more
wiley   +1 more source

Characterization of B Cell Responses in Rainbow Trout (Oncorhynchus Mykiss) Affected by Red Mark Syndrome

Advanced Science, EarlyView.
This study extensively characterizes the B cell response of rainbow trout (Oncorhynchus mykiss) in response to red mark syndrome (RMS). This disease, suspected to be caused by a Midichloria‐like organism (MLO), induces skin lesions in the infected animals, characterized by a massive influx of IgM+ B cells.
J Germán Herranz‐Jusdado, Samuel Vicente‐Gil, Esther Morel, Pablo Jiménez‐Barrios, Rocío Simón, Pedro Perdiguero, Diana Martín, Marta Vargas‐Ramírez, Niels Lorenzen, Jacob Günther Schmidt, Carolina Tafalla   +10 more
wiley   +1 more source

Analyzing human CD4+ T cells activated in response to macrophages infected with Mycobacterium tuberculosis

STAR Protocols
Summary: M1- and M2-like macrophages infected with Mycobacterium tuberculosis (Mtb) have been found to differ in their capacity to elicit memory CD4+ T cell activation. Here, we present a protocol to quantify and isolate the subset of human memory CD4+ T 
Daniel P. Gail, Vinicius G. Suzart, Stephen M. Carpenter   +2 more
doaj  

PIM1 Attenuates Innate Immunity to Foster Coronavirus Replication through Ubiquitin Ligase β‐TrCP‐Mediated IFNAR1 Degradation

Advanced Science, EarlyView.
Hijacking Host Kinase PIM1: A β‐Coronavirus (OC43) strategy to degrade IFNAR1 and evade antiviral immunity. Human β‐coronaviruses (OC43) exploit the proto‐oncoprotein PIM1 kinase to sabotage innate immunity. Viral RNA/proteins upregulate PIM1, which phosphorylates E3 ligase β‐TrCP1.
Qianya Wan, Lin Zhu, Cien Chen, Li Zhong, Houying Leung, Wei Li, Chang Xu, Xi Yao, Huan Hu, Mandi Wu, Yuxin Hou, Hin Chu, Yiran Wang, Sheng Chen, Mingyu Pan, Zongwei Cai, Ming‐liang He   +16 more
wiley   +1 more source