Results 261 to 270 of about 90,505 (292)
SPITROBOT-2 advances time-resolved cryo-trapping crystallography to under 25 ms
Spiliopoulou M +7 more
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Borate and phosphoglucose isomerase in the assay of phosphomannose isomerase
Biochimica et Biophysica Acta, 1957Abstract The isomerization catalyzed by phosphohexose isomerases is essentially irreversible in the presence of excess borate, which selectively complexes with fructose-6-phosphate. This trapping effect gives zero order kinetics as long as there is enough substrate to saturate the enzyme.
Alberto Sols, Francisco Alvarado
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Cloning and overexpression of rhamnose isomerase and fucose isomerase
Bioorganic & Medicinal Chemistry, 1995Rhamnose isomerase and fucose isomerase were overexpressed in E. coli, purified and characterized. The rhamnose isomerase gene was ligated to the restriction sites of PstI and Hind III of vector pTrcHis and the fucose isomerase gene was ligated to the EcoRI and PstI sites of vector pKK223-3 for overexpression of the enzymes in E.
Eduardo García-Junceda +3 more
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Studies on methylmalonyl isomerase
Biochimica et Biophysica Acta, 1962The partial purification (approx. 23-fold) and assay of the methylmalonyl isomerase system from bovine-liver mitochondria is described. The purified isomerase system catalyzes the isomerization of enzymically-synthesized [1-14C]- and [3-14C]methyl-malonyl-CoA to form [1-14C]- and [4-14C]succinyl-CoA, respectively. This is in agreement with the proposal
M. Daniel Lane +2 more
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Frontiers in Bioscience, 2004
Prolyl isomerases are enzymes that catalyze cis-trans isomerization of peptidyl-prolyl bonds and span three structurally unrelated protein families: the cyclophilins, FKBPs, and parvulins. The genome of the budding yeast Saccharomyces cerevisiae encodes eight different cyclophilins (Cpr1 to Cpr8), four FKBPs (Fpr1 to Fpr4), and a single parvulin (Ess1).
Xiaoyun Wu +3 more
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Prolyl isomerases are enzymes that catalyze cis-trans isomerization of peptidyl-prolyl bonds and span three structurally unrelated protein families: the cyclophilins, FKBPs, and parvulins. The genome of the budding yeast Saccharomyces cerevisiae encodes eight different cyclophilins (Cpr1 to Cpr8), four FKBPs (Fpr1 to Fpr4), and a single parvulin (Ess1).
Xiaoyun Wu +3 more
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The evolution of sugar isomerases
"Protein Engineering, Design and Selection", 1995L-Arabinose isomerase (EC 5.3.1.4) catalyzes the isomerization of L-arabinose to L-ribulose. Here we report on the purification, kinetic mechanism and chemical mechanism of L-arabinose isomerase from Escherichia coli. The enzyme catalyzes the isomerization of L-arabinose to L-ribulose by a proton transfer mechanism, in contrast to xylose isomerase ...
Soojay Banerjee +2 more
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Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2004
During the maturation of extracellular proteins, disulfide bonds that chemically cross-link specific cysteines are often added to stabilize a protein or to join it covalently to other proteins. Disulfide formation, which requires a change in the covalent structure of the protein, occurs as the protein folds into its three-dimensional structure.
Hiram F. Gilbert, Bonney Wilkinson
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During the maturation of extracellular proteins, disulfide bonds that chemically cross-link specific cysteines are often added to stabilize a protein or to join it covalently to other proteins. Disulfide formation, which requires a change in the covalent structure of the protein, occurs as the protein folds into its three-dimensional structure.
Hiram F. Gilbert, Bonney Wilkinson
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Biochemistry, 2011
Understanding the catalytic efficiency and specificity of enzymes is a fundamental question of major practical and conceptual importance in biochemistry. Although progress in biochemical and structural studies has enriched our knowledge of enzymes, the role in enzyme catalysis of residues that are not nearest neighbors of the reacting substrate ...
J. Alejandro D’Aquino +5 more
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Understanding the catalytic efficiency and specificity of enzymes is a fundamental question of major practical and conceptual importance in biochemistry. Although progress in biochemical and structural studies has enriched our knowledge of enzymes, the role in enzyme catalysis of residues that are not nearest neighbors of the reacting substrate ...
J. Alejandro D’Aquino +5 more
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Immobilization of glucose isomerase
Zeitschrift für allgemeine Mikrobiologie, 1982AbstractThe immobilization of glucose isomerase (D‐xylose ketol isomerase, EC 5.3.1.5) by covalently bonding to various carriers and by adsorption to ion exchange resins was attempted in order to obtain a stable immobilized enzyme which can be used for continuous isomerization of glucose in a column.Of the covalent bonding methods, the colloidal silica‐
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