Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae [PDF]
Frontiers in Microbiology, 2015 Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is promising approach to engineer heterologous pathway for xylose utilization in Saccharomyces cerevisiae.
Weiwen Zhang, Jian Zha, Xin Qi
exaly +4 more sources
A novel D-xylose isomerase from the gut of the wood feeding beetle Odontotaenius disjunctus efficiently expressed in Saccharomyces cerevisiae. [PDF]
Sci Rep, 2021 Carbohydrate rich substrates such as lignocellulosic hydrolysates remain one of the primary sources of potentially renewable fuel and bulk chemicals. The pentose sugar d-xylose is often present in significant amounts along with hexoses.
Silva PC +5 more
europepmc +2 more sources
Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae. [PDF]
Biotechnol Biofuels, 2021 Background Xylose contained in lignocellulosic biomass is an attractive carbon substrate for economically viable conversion to bioethanol. Extensive research has been conducted on xylose fermentation using recombinant Saccharomyces cerevisiae expressing ...
Bae JH +4 more
europepmc +2 more sources
Safety evaluation of the food enzyme xylose isomerase from the genetically modified Streptomyces rubiginosus strain DP-Pzn37. [PDF]
EFSA J, 2020 The food enzyme is a d‐xylose aldose‐ketose‐isomerase (EC 5.3.1.5) produced with the genetically modified Streptomyces rubiginosus strain DP‐Pzn37 by Danisco US Inc. Although the production strain contains antibiotic resistance genes, the food enzyme was
EFSA Panel on Food Contact Materials +28 more
europepmc +2 more sources
Engineering Xylose Isomerase and Reductase Pathways in Yarrowia lipolytica for Efficient Lipid Production. [PDF]
Microb BiotechnolXylose is a common monosaccharide in lignocellulosic residues that Yarrowia lipolytica cannot naturally metabolise for lipid production and therefore, heterologous xylose metabolic pathways must be engineered in this yeast to facilitate its consumption ...
De La Torre I +4 more
europepmc +2 more sources
Data of the crystal structure of xylose isomerase from <i>Streptomyces avermitilis</i>. [PDF]
Data BriefXylose isomerase (XI; also known as glucose isomerase) catalyzes the conversion of D-glucose and D-xylose to D-fructose and D-xylulose, respectively. XI is widely used in various industries, such as high-fructose corn syrup and bioethanol production. The
Nam KH.
europepmc +2 more sources
Decision-making in serial crystallography: a simple test to quickly determine whether sufficient data have been collected. [PDF]
Acta Crystallogr D Struct BiolWe describe a simple test to determine whether sufficient data have been collected during a serial crystallographic experiment, and its incorporation into the autoprocessing pipeline at the T‐REXX endstation on beamline P14 at the PETRA III synchrotron.In standard rotational data collection for macromolecular crystallography, data are normally ...
von Stetten D, Pearson AR.
europepmc +2 more sources
Xylose fermentation efficiency of industrial Saccharomyces cerevisiae yeast with separate or combined xylose reductase/xylitol dehydrogenase and xylose isomerase pathways. [PDF]
Biotechnol Biofuels, 2019 Background Xylose isomerase (XI) and xylose reductase/xylitol dehydrogenase (XR/XDH) pathways have been extensively used to confer xylose assimilation capacity to Saccharomyces cerevisiae and tackle one of the major bottlenecks in the attainment of ...
Cunha JT +4 more
europepmc +2 more sources
Integration of metabolic and evolutionary processes to construct efficient xylose-utilizing strain of <i>Zymomonas mobilis</i> for lignocellulosic ethanol production. [PDF]
Synth Syst BiotechnolXylose is a major component of lignocellulose, and it is crucial to enable microorganisms the capability of efficient xylose utilization for economical lignocellulosic biochemical production.
Lou J +7 more
europepmc +2 more sources
Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant
Microbial Cell Factories, 2007 Background Two heterologous pathways have been used to construct recombinant xylose-fermenting Saccharomyces cerevisiae strains: i) the xylose reductase (XR) and xylitol dehydrogenase (XDH) pathway and ii) the xylose isomerase (XI) pathway.
Hahn-Hägerdal Bärbel +3 more
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