Results 21 to 30 of about 4,843 (174)

Comparison of Isomerase and Weimberg Pathway for γ-PGA Production From Xylose by Engineered Bacillus subtilis

Frontiers in Bioengineering and Biotechnology, 2020
The production of poly-γ-glutamic acid (γ-PGA), a biopolymer consisting of D- and L-glutamic acid monomers, currently relies on L-glutamate, or citrate as carbon substrates. Here we aimed at using plant biomass-derived substrates such as xylose.
Birthe Halmschlag, Kyra Hoffmann, René Hanke, Sastia P. Putri, Eiichiro Fukusaki, Jochen Büchs, Lars M. Blank   +6 more
doaj   +1 more source

Engineering the substrate specificity of xylose isomerase [PDF]

Protein Engineering Design and Selection, 2005
Xylose isomerase (XI) catalyzes the isomerization and epimerization of hexoses, pentoses and tetroses. In order to clarify the reasons for the low reaction efficiency of a pentose sugar, L-arabinose, we determined the crystal structure of Streptomyces rubiginosus XI complexed with L-arabinose.
Johanna, Karimäki, Tarja, Parkkinen, Harri, Santa, Ossi, Pastinen, Matti, Leisola, Juha, Rouvinen, Ossi, Turunen   +6 more
openaire   +2 more sources

Genomic and phenotypic characterization of a refactored xylose-utilizing Saccharomyces cerevisiae strain for lignocellulosic biofuel production

Biotechnology for Biofuels, 2018
Background Engineered strains of Saccharomyces cerevisiae have significantly improved the prospects of biorefinery by improving the bioconversion yields in lignocellulosic bioethanol production and expanding the product profiles to include advanced ...
Phuong Tran Nguyen Hoang, Ja Kyong Ko, Gyeongtaek Gong, Youngsoon Um, Sun-Mi Lee   +4 more
doaj   +1 more source

Recombinant Diploid Saccharomyces cerevisiae Strain Development for Rapid Glucose and Xylose Co-Fermentation

Fermentation, 2018
Cost-effective production of cellulosic ethanol requires robust microorganisms for rapid co-fermentation of glucose and xylose. This study aims to develop a recombinant diploid xylose-fermenting Saccharomyces cerevisiae strain for efficient conversion of
Tingting Liu, Shuangcheng Huang, Anli Geng   +2 more
doaj   +1 more source

Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective

Microbial Cell Factories, 2017
Efficient xylose utilization is one of the most important pre-requisites for developing an economic microbial conversion process of terrestrial lignocellulosic biomass into biofuels and biochemicals.
Suryang Kwak, Yong-Su Jin
doaj   +1 more source

Impact of xylose epimerase on sugar assimilation and sensing in recombinant Saccharomyces cerevisiae carrying different xylose-utilization pathways

Biotechnology for Biofuels and Bioproducts, 2023
Background Over the last decades, many strategies to procure and improve xylose consumption in Saccharomyces cerevisiae have been reported. This includes the introduction of efficient xylose-assimilating enzymes, the improvement of xylose transport, or ...
Viktor C. Persson, Raquel Perruca Foncillas, Tegan R. Anderes, Clément Ginestet, Marie Gorwa-Grauslund   +4 more
doaj   +1 more source

Engineering and two-stage evolution of a lignocellulosic hydrolysate-tolerant Saccharomyces cerevisiae strain for anaerobic fermentation of xylose from AFEX pretreated corn stover.

PLoS ONE, 2014
The inability of the yeast Saccharomyces cerevisiae to ferment xylose effectively under anaerobic conditions is a major barrier to economical production of lignocellulosic biofuels. Although genetic approaches have enabled engineering of S. cerevisiae to
Lucas S Parreiras, Rebecca J Breuer, Ragothaman Avanasi Narasimhan, Alan J Higbee, Alex La Reau, Mary Tremaine, Li Qin, Laura B Willis, Benjamin D Bice, Brandi L Bonfert, Rebeca C Pinhancos, Allison J Balloon, Nirmal Uppugundla, Tongjun Liu, Chenlin Li, Deepti Tanjore, Irene M Ong, Haibo Li, Edward L Pohlmann, Jose Serate, Sydnor T Withers, Blake A Simmons, David B Hodge, Michael S Westphall, Joshua J Coon, Bruce E Dale, Venkatesh Balan, David H Keating, Yaoping Zhang, Robert Landick, Audrey P Gasch, Trey K Sato   +31 more
doaj   +1 more source

Metabolic engineering of Escherichia coli for the production of xylonate. [PDF]

PLoS ONE, 2013
Xylonate is a valuable chemical for versatile applications. Although the chemical synthesis route and microbial conversion pathway were established decades ago, no commercial production of xylonate has been obtained so far.
Yujin Cao, Mo Xian, Huibin Zou, Haibo Zhang   +3 more
doaj   +1 more source

Improved Plasmid-Based Inducible and Constitutive Gene Expression in Corynebacterium glutamicum

Microorganisms, 2021
Corynebacterium glutamicum has been safely used in white biotechnology for the last 60 years and the portfolio of new pathways and products is increasing rapidly.
Nadja A. Henke, Irene Krahn, Volker F. Wendisch   +2 more
doaj   +1 more source

Development and characterization of efficient xylose utilization strains of Zymomonas mobilis

Biotechnology for Biofuels, 2021
Background Efficient use of glucose and xylose is a key for the economic production of lignocellulosic biofuels and biochemicals, and different recombinant strains have been constructed for xylose utilization including those using Zymomonas mobilis as ...
Jiyun Lou, Jingwen Wang, Yongfu Yang, Qing Yang, Runxia LI, Mimi Hu, Qiaoning He, Jun Du, Xia Wang, Mian Li, Shihui Yang   +10 more
doaj   +1 more source