Results 11 to 20 of about 4,838 (170)

Comparing the xylose reductase/xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting Saccharomyces cerevisiae strains [PDF]

open access: yesBiotechnology for Biofuels, 2008
Background Ethanolic fermentation of lignocellulosic biomass is a sustainable option for the production of bioethanol. This process would greatly benefit from recombinant Saccharomyces cerevisiae strains also able to ferment, besides the hexose sugar ...
Hahn-Hägerdal Bärbel   +2 more
doaj   +3 more sources

Comparison of the xylose reductase-xylitol dehydrogenase and the xylose isomerase pathways for xylose fermentation by recombinant Saccharomyces cerevisiae [PDF]

open access: yesMicrobial Cell Factories, 2007
Background Two heterologous pathways have been used to construct recombinant xylose-fermenting Saccharomyces cerevisiae strains: i) the xylose reductase (XR) and xylitol dehydrogenase (XDH) pathway and ii) the xylose isomerase (XI) pathway.
Hahn-Hägerdal Bärbel   +3 more
doaj   +3 more sources

Immobilization, Characterization and Application of a Xylose Isomerase Biocatalyst for Xylose Fermentation in Biorefineries

open access: yesFermentation
A biocatalyst has been developed for application in the simultaneous isomerization and fermentation (SIF) of xylose, which could enable operation in repeated batches and the use of xylose from biomass hemicellulose for the production of second-generation
Márcio D. N. Ramos   +4 more
doaj   +2 more sources

Identification of Mutations Responsible for Improved Xylose Utilization in an Adapted Xylose Isomerase Expressing Saccharomyces cerevisiae Strain

open access: yesFermentation, 2022
Economic conversion of biomass to biofuels and chemicals requires efficient and complete utilization of xylose. Saccharomyces cerevisiae strains engineered for xylose utilization are still considerably limited in their overall ability to metabolize ...
Ronald E. Hector   +2 more
doaj   +1 more source

Engineering the xylose metabolism in Schizochytrium sp. to improve the utilization of lignocellulose

open access: yesBiotechnology for Biofuels and Bioproducts, 2022
Background Schizochytrium sp. is a heterotrophic, oil-producing microorganism that can efficiently produce lipids. However, the industrial production of bulk chemicals using Schizochytrium sp.
Ling-Ru Wang   +7 more
doaj   +1 more source

Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance

open access: yesBiotechnology for Biofuels, 2020
Background Efficient bioethanol production from hemicellulose feedstocks by Saccharomyces cerevisiae requires xylose utilization. Whereas S. cerevisiae does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by ...
Misun Lee   +4 more
doaj   +1 more source

Engineering the substrate specificity of xylose isomerase [PDF]

open access: yesProtein Engineering Design and Selection, 2005
Xylose isomerase (XI) catalyzes the isomerization and epimerization of hexoses, pentoses and tetroses. In order to clarify the reasons for the low reaction efficiency of a pentose sugar, L-arabinose, we determined the crystal structure of Streptomyces rubiginosus XI complexed with L-arabinose.
Johanna, Karimäki   +6 more
openaire   +2 more sources

Metabolic engineering considerations for the heterologous expression of xylose-catabolic pathways in Saccharomyces cerevisiae.

open access: yesPLoS ONE, 2020
Xylose, the second most abundant sugar in lignocellulosic biomass hydrolysates, can be fermented by Saccharomyces cerevisiae expressing one of two heterologous xylose pathways: a xylose oxidoreductase pathway and a xylose isomerase pathway.
Deokyeol Jeong   +7 more
doaj   +1 more source

Glucose Isomerase: Functions, Structures, and Applications

open access: yesApplied Sciences, 2022
Glucose isomerase (GI, also known as xylose isomerase) reversibly isomerizes D-glucose and D-xylose to D-fructose and D-xylulose, respectively. GI plays an important role in sugar metabolism, fulfilling nutritional requirements in bacteria.
Ki Hyun Nam
doaj   +1 more source

High‐yield lipid production from lignocellulosic biomass using engineered xylose‐utilizing Yarrowia lipolytica

open access: yesGCB Bioenergy, 2020
Lignocellulosic biomass shows high potential as a renewable feedstock for use in biodiesel production via microbial fermentation. Yarrowia lipolytica, an emerging oleaginous yeast, has been engineered to efficiently convert xylose, the second most ...
Sang Do Yook   +6 more
doaj   +1 more source

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