Metabolic engineering of Escherichia coli for the production of xylonate. [PDF]
PLoS ONE, 2013 Xylonate is a valuable chemical for versatile applications. Although the chemical synthesis route and microbial conversion pathway were established decades ago, no commercial production of xylonate has been obtained so far.
Yujin Cao +3 more
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Improved Plasmid-Based Inducible and Constitutive Gene Expression in Corynebacterium glutamicum
Microorganisms, 2021 Corynebacterium glutamicum has been safely used in white biotechnology for the last 60 years and the portfolio of new pathways and products is increasing rapidly.
Nadja A. Henke +2 more
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Development and characterization of efficient xylose utilization strains of Zymomonas mobilis
Biotechnology for Biofuels, 2021 Background Efficient use of glucose and xylose is a key for the economic production of lignocellulosic biofuels and biochemicals, and different recombinant strains have been constructed for xylose utilization including those using Zymomonas mobilis as ...
Jiyun Lou +10 more
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Crystallographic Studies of d-Xylose Isomerase
Journal of Biological Chemistry, 1974 In order to emphasize the similarity of the unit cell and of the diffraction patterns, this convention is only followed for Form B. The estimated error in unit cell measurements is 0.05 A for Form A crystals and 0.15 A for Form B crystals. The determined densities were reproducible within 0.02 g per cc.
H M, Berman +3 more
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Anomeric specificity of D-xylose isomerase
Biochemistry, 1992 Crystal structures of complexes of D-xylose isomerase with deoxysugars have been determined. Deoxynojirimycin is a structural analogue of alpha-pyranose and mimics the binding of these aldose substrates. The structure of this complex supports the hypothesis that an imidazole group catalyzes ring opening of the pyranose.
Collyer, C +7 more
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Xylose metabolism in the fungus Rhizopus oryzae : effect of growth and respiration on l (+)-lactic acid production [PDF]
, 2008 The fungus Rhizopus oryzae converts both glucose and xylose under aerobic conditions into chirally pure l(+)-lactic acid with by-products such as xylitol, glycerol, ethanol, carbon dioxide and fungal biomass.
Eggink, G. +7 more
core +1 more source
AMB Express, 2018 The pentose phosphate pathway (PPP) plays an important role in the synthesis of ribonucleotides and aromatic amino acids. During bioethanol production from cellulosic biomass composed mainly of d-glucose and d-xylose, the PPP is also involved in xylose ...
Yosuke Kobayashi +4 more
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Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2T [PDF]
, 2014 The xylA gene encoding xylose isomerase from Anoxybacillus gonensis G2T has been cloned and successfully expressed in E. coli. Xylose isomerase was purified 10.98-fold by heat-shock and sequential column chromatography techniques to homogeneity, and the ...
Beldüz, Ali Osman +5 more
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Reconstruction of xylose utilization pathway and regulons in Firmicutes
BMC Genomics, 2010 Background Many Firmicutes bacteria, including solvent-producing clostridia such as Clostridium acetobutylicum, are able to utilize xylose, an abundant carbon source in nature.
Zhang Weiwen +8 more
doaj +1 more source
Directed Evolution Of Xylose Isomerase For Improved Xylose Catabolism And Fermentation In The Yeast Saccharomyces Cerevisiae [PDF]
, 2012 The heterologous expression of a highly functional xylose isomerase pathway in Saccharomyces cerevisiae would have significant advantages for ethanol yield, since the pathway bypasses cofactor requirements found in the traditionally used oxidoreductase ...
Lee, Sun-Mi +2 more
core +1 more source