Results 1 to 10 of about 54 (48)

Whole‐cell studies of substrate and inhibitor specificity of isoprene monooxygenase and related enzymes [PDF]

Environmental Microbiology Reports, 2023
Abstract Co‐oxidation of a range of alkenes, dienes, and aromatic compounds by whole cells of the isoprene‐degrading bacterium Rhodococcus sp. AD45 expressing isoprene monooxygenase was investigated, revealing a relatively broad substrate specificity for this soluble diiron ...
Robin Dawson, Andrew Crombie, Laura Lehtovirta-Morley   +2 more
exaly   +4 more sources

Molecular Ecology of Isoprene-Degrading Bacteria

Microorganisms, 2020
Isoprene is a highly abundant biogenic volatile organic compound (BVOC) that is emitted to the atmosphere in amounts approximating to those of methane.
Terence Mcgenity, Ornella Carrión, Mcgenity Terry J   +2 more
exaly   +3 more sources

Novel Isoprene-Degrading Proteobacteria From Soil and Leaves Identified by Cultivation and Metagenomics Analysis of Stable Isotope Probing Experiments

Frontiers in Microbiology, 2019
Isoprene is a climate-active gas and one of the most abundant biogenic volatile organic compounds (BVOC) released into the atmosphere. In the terrestrial environment, plants are the primary producers of isoprene, releasing between 500 and 750 million ...
Terence Mcgenity, Jennifer Pratscher, Andrew Crombie   +2 more
exaly   +3 more sources

Structural and evolutionary insights into the isoprene monooxygenases

FEMS Microbiology Ecology
Abstract Isoprene, a highly reactive biogenic volatile organic compound emitted by terrestrial vegetation, influences atmospheric chemistry but its microbial degradation remains poorly understood. Aerobic degradation begins with isoprene monooxygenase (IsoMO), a multicomponent di-iron monooxygenase encoded by the isoABCDEF cluster ...
Leonardo de Oliveira Martins, Nasmille L Larke-Mejía   +1 more
exaly   +8 more sources

Isoprene Production by Sphagnum Moss Is Balanced by Microbial Uptake, as Revealed by Selective Inhibitors

Environmental Microbiology
Sphagnum moss, typical of northern peatlands, produces a range of volatile organic compounds, principally the climate‐active compound, isoprene, which serves as a carbon source for specialist isoprene‐consuming microbes. Using an inhibitor of microbial isoprene uptake, we quantify gross isoprene production by the moss and show that the vast majority of
Andrew Crombie, Laura Lehtovirta-Morley   +1 more
exaly   +2 more sources

Purification and Characterization of the Isoprene Monooxygenase from Rhodococcus sp. Strain AD45 [PDF]

Applied and Environmental Microbiology, 2022
Isoprene is a highly abundant climate-active gas and a carbon source for some bacteria. Analyses of the genes encoding isoprene monooxygenase (IsoMO) indicate this enzyme is a soluble diiron center monooxygenase in the same family of oxygenases as soluble methane monooxygenase, alkene monooxygenase, and toluene monooxygenase.
Sims, Leanne P., Lockwood, Colin W. J., Crombie, Andrew T., Bradley, Justin M., Le Brun, Nick E., Murrell, J. Colin   +5 more
openaire   +3 more sources

Genome sequencing andin silicoanalysis of isoprene degrading monooxygenase enzymes ofSphingobiumsp. BHU LFT2

Journal of Biomolecular Structure and Dynamics, 2022
The whole genome sequencing of a novel isoprene degrading strain of Sphingobium sp. BHU LFT2, its in silico analysis for identifying and characterizing enzymes, especially isoprene monooxygenases (IsoMO), which initiate the degradation process, and in vitro validation with cell extract of optimal temperature and pH and analysis for utilizing isoprene ...
Abhishek, Singh, Anand Kumar, Pandey, Suresh Kumar, Dubey   +2 more
openaire   +2 more sources

Isoprene-degrading bacteria associated with the phyllosphere of Salix fragilis, a high isoprene-emitting willow of the Northern Hemisphere

Environmental Microbiome, 2021
Background Isoprene accounts for about half of total biogenic volatile organic compound emissions globally, and as a climate active gas it plays a significant and varied role in atmospheric chemistry. Terrestrial plants are the largest source of isoprene,
Lisa Gibson, Andrew T. Crombie, Niall P. McNamara, J. Colin Murrell   +3 more
doaj   +1 more source

Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest

Biology, 2022
Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes.
Toungporn Uttarotai, Sawannee Sutheeworapong, Andrew T. Crombie, J. Colin Murrell, Wuttichai Mhuantong, Nuttapol Noirungsee, Sunanta Wangkarn, Sakunnee Bovonsombut, Terry J. McGenity, Thararat Chitov   +9 more
doaj   +1 more source

Complete Genome of Isoprene Degrading Nocardioides sp. WS12

Microorganisms, 2020
Isoprene is a climate-active gas whose wide-spread global production stems mostly from terrestrial plant emissions. The biodegradation of isoprene is carried out by a number of different bacteria from a wide range of environments. This study investigates
Lisa Gibson, Nasmille L. Larke-Mejía, J. Colin Murrell   +2 more
doaj   +1 more source