Results 121 to 130 of about 4,449 (151)
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Combining Gal4p-mediated expression enhancement and directed evolution of isoprene synthase to improve isoprene production in Saccharomyces cerevisiae

Metabolic Engineering, 2017
Current studies on microbial isoprene biosynthesis have mostly focused on regulation of the upstream mevalonic acid (MVA) or methyl-erythritol-4-phosphate (MEP) pathway. However, the downstream bottleneck restricting isoprene biosynthesis capacity caused by the weak expression and low activity of plant isoprene synthase (ISPS) under microbial ...
Pingping Zhou, Zhen Yao, Lidan Ye
exaly   +3 more sources

Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis

Biochemical and Biophysical Research Communications, 2002
Isoprene is a volatile metabolite of uncertain function in plants, animals, and bacteria. Here, we demonstrate that the isoprene-producing bacterium, Bacillus subtilis, contains an isoprene synthase activity that catalyzes dimethylallyl diphosphate-dependent isoprene formation.
Tami L, Sivy, Megan C, Shirk, Ray, Fall
openaire   +2 more sources

Biochemical properties of isoprene synthase in poplar (Populus × canescens)

Planta, 2005
Isoprene synthase (ISPS) catalyzes the elimination of pyrophosphate from dimethylallyl diphosphate (DMADP) forming isoprene, a volatile hydrocarbon emitted from many plant species to the atmosphere. In the present work, immunological techniques were applied to study and localize ISPS in poplar leaves (Populus x canescens).
Ina Zimmer   +2 more
exaly   +4 more sources

ISOPRENE SYNTHASE GENES FORM A MONOPHYLETIC CLADE OF ACYCLIC TERPENE SYNTHASES IN THE TPS-B TERPENE SYNTHASE FAMILY

Evolution; International Journal of Organic Evolution, 2013
Many plants emit significant amounts of isoprene, which is hypothesized to help leaves tolerate short episodes of high temperature. Isoprene emission is found in all major groups of land plants including mosses, ferns, gymnosperms, and angiosperms; however, within these groups isoprene emission is variable.
Thomas D Sharkey
exaly   +2 more sources

Biochemical characterization of an isoprene synthase from Campylopus introflexus (heath star moss)

Plant Physiology and Biochemistry, 2015
Each year, plants emit terragram quantities of the reactive hydrocarbon isoprene (2-methyl-1,3-butadiene) into the earth's atmosphere. In isoprene-emitting plants, the enzyme isoprene synthase (ISPS) catalyzes the production of isoprene from the isoprenoid intermediate dimethylallyl diphosphate (DMADP).
Alexandra Lantz   +2 more
exaly   +3 more sources

[Advances in isoprene synthase research].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2018
Isoprene emission can lead to significant consequence for atmospheric chemistry. In addition, isoprene is a chemical compound for various industrial applications. In the organisms, isoprene is produced by isoprene synthase that eliminates the pyrophosphate from the dimethylallyl diphosphate.
Yan, Gou   +2 more
openaire   +1 more source

Isoprene synthase activity and its relation to isoprene emission in Quercus robur L. leaves

Plant, Cell & Environment, 1999
Pedunculate oak (Quercus robur L.) is known as a strong isoprene (2‐methyl‐1,3‐butadiene) emitter. Diurnal changes in isoprene emission were determined by branch enclosure measurements. In contrast to the diurnal cycle in emission rates, specific isoprene synthase activity in the leaves remained unchanged.
A. LEHNING   +4 more
openaire   +1 more source

Enhanced Isoprene Production by Reconstruction of Metabolic Balance between Strengthened Precursor Supply and Improved Isoprene Synthase in Saccharomyces cerevisiae

ACS Synthetic Biology, 2018
Isoprene, as a versatile bulk chemical, has wide industrial applications. Here, we attempted to improve isoprene biosynthesis in Saccharomyces cerevisiae by simultaneous strengthening of precursor supply and conversion via a combination of pathway compartmentation and protein engineering. At first, a superior isoprene synthase mutant ISPSLN was created
Zhen Yao   +5 more
openaire   +2 more sources

A Novel Isoprene Synthase from the Monocot Tree Copernicia prunifera (Arecaceae) Confers Enhanced Drought Tolerance in Transgenic Arabidopsis

International Journal of Molecular Sciences, 2023
Claudio Varotto   +2 more
exaly  

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