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Isothermal Amplification of Nucleic Acids
Chemical Reviews, 2015Isothermal amplification of nucleic acids is a simple process that rapidly and efficiently accumulates nucleic acid sequences at constant temperature. Since the early 1990s, various isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR).
Yongxi, Zhao +4 more
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Ramification Amplification: A Novel Isothermal DNA Amplification Method
Molecular Diagnosis, 2001We have developed a novel isothermal DNA amplification method with an amplification mechanism quite different from conventional PCR. This method uses a specially designed circular probe (C-probe) in which the 3' and 5' ends are brought together in juxtaposition by hybridization to a target.
D Y, Zhang +3 more
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Detection of tetracycline using isothermal amplification
2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS), 2017PCR is an important biosensing method since it can amplify DNA by many orders of magnitude. However, for food safety and environmental monitoring, the target molecule is usually not DNA. Here we demonstrate a detection method that integrates competitive assay format, magnetic bead separation, and isothermal DNA amplification for sensitive detection of ...
Chia-Cheng Wu +3 more
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Quadratic isothermal amplification for the detection of microRNA
Nature Protocols, 2014This protocol describes an isothermal amplification approach for ultrasensitive detection of specific microRNAs (miRNAs). It achieves this level of sensitivity through quadratic amplification of the target oligonucleotide by using a Bst DNA polymerase-induced strand-displacement reaction and a lambda exonuclease-aided recycling reaction.
Ruixue, Duan +8 more
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Isothermal Amplification Methods for the SNP Genotyping
Current Molecular Medicine, 2019The demands for genotyping techniques with acceptable precision, accuracy, cost-effectiveness in high throughput formats made driving forces for continuous development of novel technologies. A wide range of mutation detection techniques based on polymerase chain reaction (PCR) have been introduced.
Somayeh Heidari, Sharafdarkolaee +3 more
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Isothermal DNA amplification in vitro: the helicase-dependent amplification system
Cellular and Molecular Life Sciences, 2009Since the development of polymerase chain reaction, amplification of nucleic acids has emerged as an elemental tool for molecular biology, genomics, and biotechnology. Amplification methods often use temperature cycling to exponentially amplify nucleic acids; however, isothermal amplification methods have also been developed, which do not require ...
Yong-Joo, Jeong +2 more
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SOLID PHASE ISOTHERMAL AMPLIFICATION [PDF]
, 2019 The present invention relates to an amplification technology for multiple, rapid, sensitive, specific detection of nucleic acids in samples originating from the environment, water, food, animals, plants, microorganisms, cell cultures, or humans.
WOLFF ANDERS +4 more
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[A method of isothermal gene amplification].
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie, 1999A gene amplification technique applied to the detection of HIV and which can be implemented without programmable thermocyclers is described. The reaction products are high molecular weight strands of DNA that can be detected using simple methods, even without electrophoresis. This is a promising technique for in situ amplification experiments.
F, David, E, Turlotte
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Isothermal nucleic acid amplification for food safety analysis
TrAC - Trends in Analytical Chemistry, 2022Qiang He, Ruijie Deng, Jijuan Cao
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