Results 21 to 30 of about 1,839,261 (340)
Isotope Labeling in Insect Cells [PDF]
, 2011 Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that have traditionally been difficult to study due to issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells have proved useful in allowing large quantities of isotope-
Arpana Dutta +3 more
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Bio-Protocol, 2018 Stable-isotope labeled metabolic analysis is an essential methodology to characterize metabolic regulation during biological processes. However, the method using stable-isotope-labeled tracer (e.g., 13C-glucose) in live animal is only beginning to be ...
Yuping Cai, Nan Liu, Zheng-Jiang Zhu
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BMC Bioinformatics, 2019 The investigation of intracellular metabolism is the mainstay in the biotechnology and physiology settings. Intracellular metabolic rates are commonly evaluated using labeling pattern of the identified metabolites obtained from stable isotope labeling ...
Di Du +7 more
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Chemoenzymatic Assembly of Isotopically Labeled Folates [PDF]
Journal of the American Chemical Society, 2017 Pterin-containing natural products have diverse functions in life, but an efficient and easy scheme for their in vitro synthesis is not available. Here we report a chemoenzymatic 14-step, one-pot synthesis that can be used to generate 13C- and 15N-labeled dihydrofolates (H2F) from glucose, guanine, and p-aminobenzoyl-l-glutamic acid.
Antonio Angelastro +4 more
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An origin identification model for labeling of shiitake (Lentinula edodes)
npj Science of Food, 2021 With the increasing globalization of the food trade across countries and continents, reliable identification of the geographical origin of products is critical. In this study, we describe the limitations of the current origin labeling system for non-soil-
Ill-Min Chung +6 more
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Scientific Reports, 2018 Experiments with stable isotope tracers such as 13C and 15N are increasingly used to gain insights into metabolism. However, mass spectrometric measurements of stable isotope labeling experiments should be corrected for the presence of naturally ...
Paul Heinrich +6 more
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Synthesis of isotopically labelled ubiquinones
Tetrahedron Letters, 1992 Abstract Ubiquinones ( 1 ) of various chain lengths have been synthesized. The key step involves the condensation of a long chain polyunsaturated alcohol onto an aromatic intermediate. The synthetic methodology allows for the incorporation of isotopic labels in the methoxy groups attached to the ring portion of the molecule.
Duralski, A, Watts, A
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Synthesis of Stable Isotope Deuterium Labeled Levofloxacin-D3
Journal of Isotopes, 2023 In order to improve the stable isotope utilization rate, a simple and inexpensive synthesis method was developed. The synthesis of levofloxacin is based on piperazine as the starting material, and the reaction with carbobenzoxy chloride (CBZ-Cl) to get ...
XU Zhong jie;ZHANG Li;SUN Wen;ZHANG Pengshuai;LEI Wen
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Limitations of In-Vitro Labeling of Endothelial Cells with Indium-111 Oxine
Cell Transplantation, 1995 Indium-111 oxine labeling is widely used as a marker of endothelial cell attachment to vascular prostheses. The long term effect of labeling human adult endothelial cells (HAECs) with this isotope has not been determined.
H.M.H. Carr +5 more
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Synthesis of Sulfachloropyridazine-Benzene Ring-13C6
Journal of Isotopes, 2023 A synthesis route for stable isotope-labeled sulfamethoxazole-benzene ring-13C6 has been developed. In this strategy, 13C6-benzene was used as the source and starting material for stable isotope labeling and starting material to synthesize ...
MEI Shu;GUO Hui;CHEN Wulian
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