Structural and biochemical analysis of a B12 superbinder
FEBS Letters, EarlyView.BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Improving the measurement of QALYs in dementia: developing patient- and carer-reported health state classification systems using Rasch analysis [PDF]
Objectives: Cost-utility analysis is increasingly used to inform resource allocation. This requires a means of valuing health states before and after intervention. Although generic measures are typically used to generate values, these do not perform well
Knapp, M +9 more
core
Impact of word properties on list learning: An explanatory item analysis. [PDF]
Neuropsychology, 2023 Goette WF +6 more
europepmc +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
Minimum accepted competency examination: test item analysis. [PDF]
BMC Med Educ, 2022 McCrossan P, Nicholson A, McCallion N.
europepmc +1 more source
FEBS Letters, EarlyView.How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley +1 more source
Una prova di abilità linguistiche per l’uscita dai percorsi di Formazione professionale
Journal of Educational, Cultural and Psychological Studies, 2010 A Language Competence Test on Exiting Vocational Training Programmes This paper illustrates the methodology adopted to design and calibrate a test for measuring students’ language skills at the end of the third year of vocational training courses (10th ...
Pietro Lucisano
doaj
Predicting tobacco product initiation from intentions to use: Comparing the validity of item analysis methods. [PDF]
Prev Med Rep, 2022 Persoskie A, O'Brien EK.
europepmc +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Cross-cultural adaptation, content validity, and item analysis of the Objective and Subjective Knowledge and HIV Testing Scale for the Brazilian population. [PDF]
Trends Psychiatry Psychother, 2023 Silva RA +3 more
europepmc +1 more source