Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
A Bayesian Generalized Explanatory Item Response Model to Account for Learning During the Test. [PDF]
Psychometrika, 2021 Lozano JH, Revuelta J.
europepmc +1 more source
Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing
FEBS Letters, EarlyView.Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley +1 more source
LNIRT: LogNormal Response Time Item Response Theory Models [PDF]
, 2016 Jean-Paul Fox, Konrad Klotzke, Rinke Klein Entink
openalex +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
eirm: Explanatory Item Response Modeling for Dichotomous and Polytomous Items
, 2021 Okan
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Function‐driven design of a surrogate interleukin‐2 receptor ligand
FEBS Letters, EarlyView.Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang +9 more
wiley +1 more source
PRACTICAL APPLICATIONS OF POSTERIOR PREDICTIVE MODEL CHECKING FOR ASSESSING FIT OF COMMON ITEM RESPONSE THEORY MODELS [PDF]
, 2003 Sandip Sinharay
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RASCH MODELLING TO EVALUATE REASONING DIFFICULTIES, CHANGES OF RESPONSES, AND ITEM MISCONCEPTION PATTERN OF HYDROLYSIS [PDF]
, 2022 Lukman Abdul Rauf Laliyo +5 more
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Modeling Local Item Dependencies in Item Response Theory
Psychologica Belgica, 1998 In this paper, an introduction to some existing Item Response Theory (IRT) models for Local Item Dependencies (LIDs) is presented together with an application. First, four LID models introduced by Hoskens and De Boeck (1997) are discussed and a more general model is derived. Second, it is described in broad outline how these models can be estimated and
Tuerlinckx, Francis, De Boeck, Paul
openaire +3 more sources