Results 221 to 230 of about 37,289 (238)
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Nematology, 2005
AbstractDifferentiation of the plant-pathogenic pinewood nematode, Bursaphelenchus xylophilus, from non-pathogenic Bursaphelenchus species is difficult because of high morphological similarities among closely related species. In recent years, ITS-RFLP analysis has become a useful tool for Bursaphelenchus species identification.
Wolfgang Burgermeister, Kai Metge
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AbstractDifferentiation of the plant-pathogenic pinewood nematode, Bursaphelenchus xylophilus, from non-pathogenic Bursaphelenchus species is difficult because of high morphological similarities among closely related species. In recent years, ITS-RFLP analysis has become a useful tool for Bursaphelenchus species identification.
Wolfgang Burgermeister, Kai Metge
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Mycological Research, 2005
We used ITS-RFLP and sequence analysis to determine the identities of the fungal endophytes of six terrestrial orchid species from southeastern Queensland, a region previously unexplored in this context. Pure cultures of orchid--colonising fungi were obtained and fungal identities were assessed by means of ITS-PCR, RFLP analysis, sequence comparison ...
Bougoure, Jeremy J. +3 more
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We used ITS-RFLP and sequence analysis to determine the identities of the fungal endophytes of six terrestrial orchid species from southeastern Queensland, a region previously unexplored in this context. Pure cultures of orchid--colonising fungi were obtained and fungal identities were assessed by means of ITS-PCR, RFLP analysis, sequence comparison ...
Bougoure, Jeremy J. +3 more
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Mycological Research, 2001
Fifty-three isolates of Pisolithus were obtained from various locations in central and eastern Australia. These, along with two isolates from south-east Asia and one from USA, were compared using ITS–RFLP and ITS sequencing analyses. Results of the RFLP analysis initially grouped the isolates into eight RFLP types.
Anderson, Ian C. +2 more
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Fifty-three isolates of Pisolithus were obtained from various locations in central and eastern Australia. These, along with two isolates from south-east Asia and one from USA, were compared using ITS–RFLP and ITS sequencing analyses. Results of the RFLP analysis initially grouped the isolates into eight RFLP types.
Anderson, Ian C. +2 more
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Ectomycorrhizal community composition of Pinus tabulaeformis assessed by ITS-RFLP and ITS sequences
Botany, 2010Ectomycorrhizal (ECM) fungal composition was examined in a Pinus tabulaeformis Carr. forest. A total of 28 root samples of P. tabulaeformis were collected in June and September. Thirty-five ECM morphotypes were identified according to ECM morphological characters, and 26 ECM fungi were identified based on the analyses of ITS-RFLP and ITS sequences ...
Qin Wang, Liang-Dong Guo
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European Food Research and Technology, 2009
In the present study, ochratoxigenic mycobiota in cocoa beans was identified at species level by digestion of the ITS products using the endonucleases HhaI, NlaIII and RsaI. Of the 132 isolates of Aspergillus section Nigri collected from cocoa beans, 89 were identified as A. tubingensis, 27 as A. niger, 10 as A. tubingensis-like and 6 as A. carbonarius.
Fatima Bisbal +3 more
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In the present study, ochratoxigenic mycobiota in cocoa beans was identified at species level by digestion of the ITS products using the endonucleases HhaI, NlaIII and RsaI. Of the 132 isolates of Aspergillus section Nigri collected from cocoa beans, 89 were identified as A. tubingensis, 27 as A. niger, 10 as A. tubingensis-like and 6 as A. carbonarius.
Fatima Bisbal +3 more
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Journal of Forest Research, 2013
Analysis of the restriction fragment length polymorphism of the internal transcribed spacer region of ribosomal DNA (ITS-RFLP) is an efficient tool for classifying Bursaphelenchus nematodes at or below the species level. In B. xylophilus, it has been shown that the ITS-RFLP pattern of Japanese avirulent (nonpathogenic) isolates is different from that ...
Takuya Aikawa +2 more
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Analysis of the restriction fragment length polymorphism of the internal transcribed spacer region of ribosomal DNA (ITS-RFLP) is an efficient tool for classifying Bursaphelenchus nematodes at or below the species level. In B. xylophilus, it has been shown that the ITS-RFLP pattern of Japanese avirulent (nonpathogenic) isolates is different from that ...
Takuya Aikawa +2 more
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International Journal of Food Microbiology, 2007
Ochratoxigenic mycobiota in grapes from representative wine regions in Valencia was identified. Black aspergilli were predominant among the different Aspergillus spp. isolated. Restriction digestion analysis of the ITS products was tested as a rapid method to identify isolates of black Aspergillus species from grapes. Restriction endonuclease digestion
P V, Martínez-Culebras, D, Ramón
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Ochratoxigenic mycobiota in grapes from representative wine regions in Valencia was identified. Black aspergilli were predominant among the different Aspergillus spp. isolated. Restriction digestion analysis of the ITS products was tested as a rapid method to identify isolates of black Aspergillus species from grapes. Restriction endonuclease digestion
P V, Martínez-Culebras, D, Ramón
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Parasitology Research, 2009
Chagas disease is a severe public health problem in Latin-American countries. In Colombia, the predominance of Trypanosoma cruzi I has been described in the literature, with a broad heterogeneity between strains. However, most of the studies carried out centered on isoenzyme analysis, with a smaller number that focus on other molecular methods. In this
Katherine Paola, Luna-Marín +5 more
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Chagas disease is a severe public health problem in Latin-American countries. In Colombia, the predominance of Trypanosoma cruzi I has been described in the literature, with a broad heterogeneity between strains. However, most of the studies carried out centered on isoenzyme analysis, with a smaller number that focus on other molecular methods. In this
Katherine Paola, Luna-Marín +5 more
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World Journal of Microbiology and Biotechnology, 2011
Sclerotinia sclerotiorum is one of the most devastating soil-inhabiting fungal plant pathogens infecting various crop plants including chickpea. Genetic diversity of 24 isolates of S. sclerotiorum representing 10 different states of India was determined by different molecular markers and mycelial compatibility grouping (MCG).
A K, Mandal, Sunil C, Dubey
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Sclerotinia sclerotiorum is one of the most devastating soil-inhabiting fungal plant pathogens infecting various crop plants including chickpea. Genetic diversity of 24 isolates of S. sclerotiorum representing 10 different states of India was determined by different molecular markers and mycelial compatibility grouping (MCG).
A K, Mandal, Sunil C, Dubey
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Correlation between ITS-RFLP & sequencing for Staphylococcus Aureus typing in Lebanon. (c2009)
2017Includes bibliographical references (leaves 39-53). ; Staplylocol'l'UJ' aureus has been associated with a wide range of serious communityand hospital-related infections worldwide. The molecular characterization of S. aureus is of both clinical and infection control importance. In this context we studied the virulence determinants among 130 S.
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