Results 41 to 50 of about 794,230 (296)

Homology-mediated end joining-based targeted integration using CRISPR/Cas9

Cell Research, 2017
Targeted integration of transgenes can be achieved by strategies based on homologous recombination (HR), microhomology-mediated end joining (MMEJ) or non-homologous end joining (NHEJ).
Xuan Yao, Xing Wang, Xinde Hu, Zhen Liu, Junlai Liu, Haibo Zhou, Xiaowen Shen, Yu Wei, Zijian Huang, Wenqin Ying, Yan Wang, Yanhong Nie, Chenchen Zhang, Sanlan Li, Le-ping Cheng, Qifang Wang, Yan Wu, Pengyu Huang, Qiang Sun, Linyu Shi, Hui Yang   +20 more
semanticscholar   +1 more source

Mapping the evolution of mitochondrial complex I through structural variation

FEBS Letters, EarlyView.
Respiratory complex I (CI) is crucial for bioenergetic metabolism in many prokaryotes and eukaryotes. It is composed of a conserved set of core subunits and additional accessory subunits that vary depending on the organism. Here, we categorize CI subunits from available structures to map the evolution of CI across eukaryotes. Respiratory complex I (CI)
Dong‐Woo Shin, Tingting Chen, James A. Letts   +2 more
wiley   +1 more source

Joining Technology Innovations at the Macro, Micro, and Nano Levels

Applied Sciences, 2019
With the growing joining requirements of emergent engineering materials and new applications, conventional welding continues to evolve at all scales spanning from the macro- down to the micro- and nanoscale.
Anming Hu, Jolanta Janczak-Rusch, Tomokazu Sano   +2 more
doaj   +1 more source

Investigation of the Pull-Out Behaviour of Metal Threaded Inserts in Thermoplastic Fused-Layer Modelling (FLM) Components

Journal of Manufacturing and Materials Processing, 2023
To provide detachable, secure and long-term stable joints in fused-layer modelling (FLM) components or assemblies, metal threaded inserts are widely used as extrinsic interfaces.
Tobias Kastner, Juliane Troschitz, Christian Vogel, Thomas Behnisch, Maik Gude, Niels Modler   +5 more
doaj   +1 more source

Repair of DNA double-strand breaks by mammalian alternative end-joining pathways

Journal of Biological Chemistry, 2018
Alternative end-joining (a-EJ) pathways, which repair DNA double-strand breaks (DSBs), are initiated by end resection that generates 3′ single strands. This reaction is shared, at least in part, with homologous recombination but distinguishes a-EJ from ...
A. Sallmyr, A. Tomkinson
semanticscholar   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

Molecular basis of microhomology-mediated end-joining by purified full-length Polθ

Nature Communications, 2019
DNA polymerase θ (Polθ) is a unique polymerase-helicase fusion protein that promotes microhomology-mediated end-joining (MMEJ) of DNA double-strand breaks (DSBs). How full-length human Polθ performs MMEJ at the molecular level remains unknown.
S. Black, Ahmet Y. Ozdemir, E. Kashkina, T. Kent, Timur Rusanov, D. Ristic, Y. Shin, A. Suma, Trung M. Hoang, Gurushankar Chandramouly, Labiba A. Siddique, Nikita Borisonnik, Katherine Sullivan-Reed, Joseph S. Mallon, T. Skorski, V. Carnevale, K. Murakami, C. Wyman, R. Pomerantz   +18 more
semanticscholar   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

The role of histone modifications in transcription regulation upon DNA damage

FEBS Letters, EarlyView.
This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley   +1 more source