Results 21 to 30 of about 98,997 (145)

Genome-wide identification of bacterial genes contributing to nucleus-forming jumbo phage infection. [PDF]

Nucleic Acids Res
The Chimalliviridae family of bacteriophages (phages) form a proteinaceous nucleus-like structure during infection of their bacterial hosts. This phage ‘nucleus’ compartmentalises phage DNA replication and transcription, and shields the phage genome from
Harding KR, Malone LM, Kyte NAP, Jackson SA, Smith LM, Fineran PC.   +5 more
europepmc   +4 more sources

Jumbo phage killer immune system targets early infection of nucleus-forming phages. [PDF]

Cell
Jumbo bacteriophages of the ϕKZ-like family assemble a lipid-based early phage infection (EPI) vesicle and a proteinaceous nucleus-like structure during infection. These structures protect the phage from nucleases and may create selective pressure for immunity mechanisms targeting this specific phage family. Here, we identify "jumbo phage killer" (Juk),
Yuping L, Guan L, Becher I, Makarova KS, Cao X, Hareendranath S, Guan J, Stein F, Yang S, Boergel A, Lapouge K, Remans K, Agard D, Savitski M, Typas A, Koonin EV, Feng Y, Bondy-Denomy J.   +17 more
europepmc   +4 more sources

Exploring the enzymatic activity of depolymerase gp531 from Klebsiella pneumoniae jumbo phage RaK2. [PDF]

Virus Res, 2023
Klebsiella pneumoniae poses a major global challenge due to its virulence, multidrug resistance, and nosocomial nature. Thus, bacteriophage-derived proteins are extensively being investigated as a means to combat this bacterium. In this study, we explored the enzymatic specificity of depolymerase gp531, encoded by the jumbo bacteriophage vB_KleM_RaK2 ...
Noreika A, Stankevičiūtė J, Rutkienė R, Meškys R, Kalinienė L.   +4 more
europepmc   +6 more sources

A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli. [PDF]

Cell Rep, 2022
AbstractVortex-like arrays of cytoskeletal filaments that drive cytoplasmic streaming and nucleus rotation have been identified in eukaryotes, but similar structures have not been described in prokaryotes. The only known example of a rotating intracellular body in prokaryotic cells occurs when nucleus-forming jumbo phages infect Pseudomonas.
Birkholz EA, Laughlin TG, Armbruster E, Suslov S, Lee J, Wittmann J, Corbett KD, Villa E, Pogliano J.   +8 more
europepmc   +6 more sources

Characterisation of a Novel Jumbo Lytic Aeromonas dhakensis Bacteriophage P19 and Its Endolysin [PDF]

Microbial Biotechnology
Aeromonas dhakensis is an emerging multidrug‐resistant (MDR) bacterial pathogen that presents significant threats to public health and aquaculture. This study reports the isolation and comprehensive characterisation of jumbo bacteriophage P19.
Farhat Ansari, Vandan Nagar
doaj   +3 more sources

Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection. [PDF]

Cell Host Microbe
Viral genomes are most vulnerable to cellular defenses at the start of the infection. A family of jumbo phages related to phage ΦKZ, which infects Pseudomonas aeruginosa, assembles a protein-based phage nucleus to protect replicating phage DNA, but how it is protected prior to phage nucleus assembly is unclear.
Mozumdar D, Fossati A, Stevenson E, Guan J, Nieweglowska E, Rao S, Agard D, Swaney DL, Bondy-Denomy J.   +8 more
europepmc   +5 more sources

Characterization and genome analysis of jumbo Escherichia phage UE-S1 and the antibacterial potential of its lysozyme against uropathogenic E. coli [PDF]

BMC Microbiology
Background Every year, millions of individuals worldwide are affected by urinary tract infections (UTIs), one of the most frequent bacterial infections.
Hira Niaz, Anza Abbas, Aqsa Ayub, Abdur Rahman, Fazal Adnan   +4 more
doaj   +3 more sources

Molecular basis for anti-jumbo phage immunity by AVAST Type 5

bioRxiv
Abstract Jumbo phages protect their genomes from DNA-sensing bacterial defense systems by enclosing them within vesicles and nucleus-like compartments. Very little is known about defense systems specialized to counter these phages. Here, we show that AVAST Type 5 (Avs5) systems, part of the STAND superfamily and spanning three phylogenetic ...
Muralidharan A, Costa AR, Fierlier D, van den Berg DF, van den Bossche H, Zoumaro-Djayoon AD, Pabst M, Pacesa M, Correia BE, Brouns SJJ.   +9 more
europepmc   +4 more sources

Immediate targeting of host ribosomes by jumbo phage encoded proteins

bioRxiv, 2023
AbstractBacteriophages must seize control of the host gene expression machinery to promote their own protein synthesis. Since the bacterial hosts are armed with numerous anti-phage defence systems, it is essential that mechanisms of host take-over act immediately upon infection.
Gerovac M, Chihara K, Wicke L, Böttcher B, Lavigne R, Vogel J.   +5 more
europepmc   +3 more sources

Complete genome sequence of a <i>Ralstonia</i>-infecting jumbo phage isolated from Maryland, USA. [PDF]

Microbiol Resour Announc
A Ralstonia-infecting jumbo phage was isolated from soil in Maryland, USA. The complete genome sequence was determined to be 225,638 bp and has a G+C content of 51.8%.
Shao J, Ahmad AA, Huang Q.
europepmc   +2 more sources