Results 21 to 30 of about 871 (146)

A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli. [PDF]

Cell Rep, 2022
Nucleus-forming jumbo phages establish an intricate subcellular organization, enclosing phage genomes within a proteinaceous shell called the phage nucleus.
Birkholz EA, Laughlin TG, Armbruster E, Suslov S, Lee J, Wittmann J, Corbett KD, Villa E, Pogliano J.   +8 more
europepmc   +6 more sources

Jumbo phage–mediated transduction of genomic islands [PDF]

Proceedings of the National Academy of Sciences of the United States of America
Abstract Bacteria acquire new genes by horizontal gene transfer (HGT), typically mediated by mobile genetic elements (MGEs). While plasmids, bacteriophages and certain integrative and conjugative elements (ICEs) are well characterized, the broader diversity of MGEs remains poorly understood.
Yansong Zhao, Christina Vasileiou, Andrew D Farr   +2 more
exaly   +8 more sources

Jumbo phage killer immune system targets early infection of nucleus-forming phages [PDF]

Cell
Jumbo bacteriophages of the ϕKZ-like family assemble a lipid-based early phage infection (EPI) vesicle and a proteinaceous nucleus-like structure during infection. These structures protect the phage from nucleases and may create selective pressure for immunity mechanisms targeting this specific phage family. Here, we identify "jumbo phage killer" (Juk),
Xueli Cao
exaly   +4 more sources

Genomic and proteomic characterization of vB_SauM-UFV_DC4, a novel Staphylococcus jumbo phage. [PDF]

Appl Microbiol Biotechnol, 2023
Staphylococcus aureus is one of the most relevant mastitis pathogens in dairy cattle, and the acquisition of antimicrobial resistance genes presents a significant health issue in both veterinary and human fields.
da Silva JD, Melo LDR, Santos SB, Kropinski AM, Xisto MF, Dias RS, da Silva Paes I, Vieira MS, Soares JJF, Porcellato D, da Silva Duarte V, de Paula SO.   +11 more
europepmc   +5 more sources

Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection [PDF]

Cell Host and Microbe
Viral genomes are most vulnerable to cellular defenses at the start of the infection. A family of jumbo phages related to phage ΦKZ, which infects Pseudomonas aeruginosa, assembles a protein-based phage nucleus to protect replicating phage DNA, but how it is protected prior to phage nucleus assembly is unclear.
Deepto Mozumdar, Andrea Fossati, Erica Stevenson   +2 more
exaly   +5 more sources

A phage nucleus-associated RNA-binding protein is required for jumbo phage infection. [PDF]

Nucleic Acids Res
Large-genome bacteriophages (jumbo phages) of the proposed family Chimalliviridae assemble a nucleus-like compartment bounded by a protein shell that protects the replicating phage genome from host-encoded restriction enzymes and DNA-targeting CRISPR-Cas
Enustun E, Armbruster EG, Lee J, Zhang S, Yee BA, Malukhina K, Gu Y, Deep A, Naritomi JT, Liang Q, Aigner S, Adler BA, Cress BF, Doudna JA, Chaikeeratisak V, Cleveland DW, Ghassemian M, Bintu B, Yeo GW, Pogliano J, Corbett KD.   +20 more
europepmc   +6 more sources

Capsid structure of bacteriophage ΦKZ provides insights into assembly and stabilization of jumbo phages

Nature Communications
Jumbo phages are a group of tailed bacteriophages with large genomes and capsids. As a prototype of jumbo phage, ΦKZ infects Pseudomonas aeruginosa, a multi-drug-resistant (MDR) opportunistic pathogen leading to acute or chronic infection in ...
Yashan Yang, Qianqian Shao, Mingcheng Guo, Lin Han, Xinyue Zhao, Aohan Wang, Xiangyun Li, Bo Wang, Ji-An Pan, Zhenguo Chen, Andrei Fokine, Lei Sun, Qianglin Fang   +12 more
doaj   +4 more sources

Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage. [PDF]

Sci Adv, 2022
Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages are generally assumed to assemble randomly throughout the host cell cytoplasm.
Chaikeeratisak V, Khanna K, Nguyen KT, Egan ME, Enustun E, Armbruster E, Lee J, Pogliano K, Villa E, Pogliano J.   +9 more
europepmc   +6 more sources

Multisubunit RNA Polymerases of Jumbo Bacteriophages

Viruses, 2020
Prokaryotic viruses with DNA genome longer than 200 kb are collectively referred to as “jumbo phages”. Some representatives of this phylogenetically diverse group encode two DNA-dependent RNA polymerases (RNAPs)—a virion RNAP and a non-virion RNAP.
Maria L. Sokolova, Inna Misovetc, Konstantin V. Severinov   +2 more
doaj   +2 more sources

Isolation protocol of jumbo phage from winter grass soil [PDF]

IOP Conference Series: Earth and Environmental Science, 2021
Jumbo phage can isolated by two different methods and protocols. It depends on size, phage species, substrate sources, environment, media and tools. Each method has positive and negative results within each factors.
T KAWASAKI, T Kawasaki, SAAD, A Mohamed, WAFA, Ali, ADDY, H S   +3 more
core   +2 more sources