Results 21 to 30 of about 192 (134)
The Transcription Factor FoxO3a Inhibits Proliferation, Promotes Apotosis and Differerntiation of K562 Cell [PDF]
, 2012 慢性粒细胞白血病一旦发展至急变期，预后极差，目前的各种治疗方法均不能有效控制其病情进展，多在数月内死亡，因而加强对急变期的研究具有很重要的科学及临床意义。急变发生机制中，BCR-ABL融合基因大量表达、继发性多种基因突变以及凋亡受阻、耐药等机制逐渐已经被阐明，但有关分化障碍的机制研究很少且不明确[1;2]。分化障碍往往与信号通路中的转录因子异常有关，FoxO3a是FOX转录因子家族是一个重要成员，是BCR-ABL/PI3K/AKT信号通路的下游因子，而其在慢粒急变中的功能失活 ...
李楠楠
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Rare Metals, EarlyView.ABSTRACT Lung cancer remains the leading cause of cancer‐related deaths worldwide, yet immune checkpoint blockade (ICB) is often compromised by an immunosuppressive tumor microenvironment (TME) with acidosis, hypoxia, and poor T cell infiltration. Here, we develop a TME‐responsive nanoplatform (MgH2@Lip/PD‐L1) that couples hydrogen gas therapy with PD ...
Xuyu Gu +5 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2008 目的探讨姜黄素诱导人红白血病细胞（K562）凋亡机制及其对细胞周期各时相变化。方法应用噻唑蓝（MTT）比色法和流式细胞术检测不同浓度的姜黄素对K562细胞增殖抑制率。细胞周期及凋亡率变化,并通过电子显微镜观察其亚细胞结构改变。结果姜黄素对K562有明显的增殖抑制作用,且呈剂量依赖性,流式细胞术结果显示,G1期细胞增多,S期细胞减少,G2/M期细胞相对增多,电子显微镜结果显示,细胞空化,线粒体肿胀,染色质趋边凝集。结论姜黄素对K562细胞有明显的抑制作用,阻止G1期细胞向S期细胞转化进程 ...
回学军 +4 more
doaj
Antitumor activity of diterpenoids from Solidago canadensis L(加拿大一枝黄花二萜成分的抗肿瘤活性)
Zhejiang Daxue xuebao. Lixue ban, 2007 通过对4株人癌细胞系，肝癌SMMC 7721，红白血病K562、乳腺癌Bcap 37和肺腺癌SPC-A1的体外抑制实验发现，加拿大一枝黄花花序的石油醚、乙酸乙酯浸膏具有较强的体外抑制肿瘤细胞生长的能力.二者对上述4株肿瘤细胞的半数抑制浓度（IC50）低于50 µg/mL.依据初筛结果，采用MTT法进行活性成分的跟踪检测，从乙酸乙酯浸膏中分离到两个抗肿瘤活性二萜.由NMR确定它们的结构分别是6β-当归酰克拉文酸和6β-巴豆酰克拉文酸.这两个化合物对4株肿瘤细胞株的半数抑制浓度（IC50）在7~12 µg ...
LIUXiao-yue(刘晓月) +5 more
doaj +1 more source
Functional Ecology, EarlyView.Read the free Plain Language Summary for this article on the Journal blog. Abstract Organic phosphorus mineralization is a critical process in the phosphorus cycle, governing phosphorus bioavailability for plants. The PhoD gene, which encodes the key enzyme alkaline phosphatase, serves as a valuable biomarker for this process.
Sandhya Mishra +3 more
wiley +1 more source
Use of a Cytobrush for Sampling the Ear Canal of Dogs With Otitis Externa
Veterinary Dermatology, EarlyView.Cytological examination of the ear canal is essential for evaluating dogs with otitis externa (OE). The conventional sampling method uses a swab. However, the cytobrush (gynaecological cervical brush), already used for cytological collection from other anatomical sites, has not been adequately investigated for this purpose in dogs with OE.
Nicoly Radaeli Atanasio +3 more
wiley +1 more source
Zhongguo shiyan zhenduanxue, 2007 目的探讨K562细胞多重耐药性形成的分子机制。方法采用MTT法分别测定K562及阿霉素耐药的K562细胞对顺铂的耐药程度,采用RT-PCR检测细胞表面MDR、MRP、GST及MGMT分子表达水平,并确定其与耐药性的关系。结果顺铂对K562细胞的抑制率明显高于阿霉素耐药的K562细胞,同时阿霉素耐药的K562细胞的表面MDR、MRP、GST及MGMT的表达明显增高。结论MDR、MRP、GST及MGMT的高表达是K562细胞形成多重耐药性的重要机制之一。
杨冬艳 +5 more
doaj
Research on the effect of DHA on glucose metabolism in human lung cancer cells and its anti-tumor mechanism [PDF]
, 2015 研究背景及目的： 肺癌是人类最常见的恶性肿瘤之一，尽管近年来治疗取得了很大进展，治疗效果仍然较差。癌细胞具有偏向通过糖酵解方式来进行糖代谢获得能量的代谢特征使得肿瘤细胞能够比正常细胞具有更强的葡萄糖代谢能力，为肿瘤细胞的快速增殖与生长提供了能量来源，也使其具备更强的凋亡抵抗能力。天然产物凭借其来源广泛、类型多样、不良反应小，具有开发为化疗药物增效减毒辅助药物的潜力等特点备受关注。双氢青蒿素是青蒿叶中分离的有效抗疟成分，体内代谢活性产物为双氢青蒿素。药物学研究证实青蒿素类药物具有抗疟、抗血吸虫 ...
米彦军
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Veterinary Dermatology, EarlyView.Background: DNA‐based vaccination rapidly induces strong cellular and humoral immune responses, which may be enhanced by inclusion of lysosomal‐associated membrane protein‐1 (LAMP). Objectives: This proof‐of‐concept study evaluated the efficacy and safety of a Der f 2/Zen 1‐LAMP‐based DNA vaccine immunotherapy in client‐owned dogs with nonseasonal AD ...
Petra Bizikova +6 more
wiley +1 more source
肿瘤治疗的新途径——中药有效成分诱导恶性肿瘤细胞分化 [PDF]
, 2002 中药有效成分诱导肿瘤细胞分化是肿瘤细胞诱导分化治疗发展的一个新的领域。区别于直接杀灭肿瘤细胞的疗法 ,对机体正常细胞的负影响较小 ,并在毒副作用等问题上优于传统中药。其药理机制是从抑制癌基因等多种途径作用 ...
李祺福, 李鹏, 黄胤怡
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