Results 101 to 110 of about 79,611 (233)

K562细胞多重耐药机制的实验研究

Zhongguo shiyan zhenduanxue, 2007
目的探讨K562细胞多重耐药性形成的分子机制。方法采用MTT法分别测定K562及阿霉素耐药的K562细胞对顺铂的耐药程度,采用RT-PCR检测细胞表面MDR、MRP、GST及MGMT分子表达水平,并确定其与耐药性的关系。结果顺铂对K562细胞的抑制率明显高于阿霉素耐药的K562细胞,同时阿霉素耐药的K562细胞的表面MDR、MRP、GST及MGMT的表达明显增高。结论MDR、MRP、GST及MGMT的高表达是K562细胞形成多重耐药性的重要机制之一。
杨冬艳, 康劲松, 孔晓霞, 张宏宇, 高凤彤, 王辉   +5 more
doaj  

Mn-Zn ferrite nanoparticles inducing ferroptosis to reverse the resistance in CML cells

Journal of Translational Medicine
Background Chronic myeloid leukemia (CML) is a malignant clonal disease of hematopoietic stem cells driven by the BCR-ABL fusion gene. Although tyrosine kinase inhibitors (TKIs) serve as targeted therapies, drug resistance remains one of the key ...
Miao Zhu, Yanqian Zhao, Li Xu, Mei Sun, Yuxiang Sun   +4 more
doaj   +1 more source

Revealing nascent proteomics in signaling pathways and cell differentiation. [PDF]

, 2018
Regulation of gene expression at the level of protein synthesis is a crucial element in driving how the genetic landscape is expressed. However, we are still limited in technologies that can quantitatively capture the immediate proteomic changes that ...
Burlingame, Alma L, Chalkley, Robert J, Forester, Craig M, Oses-Prieto, Juan A, Phillips, Nancy J, Ruggero, Davide, Urisman, Anatoly, Zhang, Li, Zhao, Qian   +8 more
core   +1 more source

Quantitative analysis of DNA‐GATA1 binding alterations linked to hematopoietic disorders

The FEBS Journal, EarlyView.
Native holdup allows the quantitative determination of affinities between full‐length transcription factors and DNA. Mutations in either the protein or the DNA can modulate binding strength, which can be precisely quantified using this approach. Applied to GATA1, it revealed mutations that alter DNA binding.
Boglarka Zambo, Bastien Morlet, Luc Negroni, Orsolya Mózner, Balázs Sarkadi, Gilles Travé, Gergo Gogl   +6 more
wiley   +1 more source

AlkaPlorer: A database‐driven explorer for natural alkaloids and derivatives

Journal of Integrative Plant Biology, EarlyView.
The alkaloid database AlkaPlorer integrates over 130,000 compounds across 12,250 species. By linking chemical structures with biological and evolutionary data, it reveals how these molecules evolve and function. This platform serves as a vital resource for AI‐driven discovery in plant metabolism and modern drug development. ABSTRACT Alkaloids, renowned
Jiahao Li, Tao Zeng, Hongquan Xu, Xu Kang, Minghui Liang, Ruibo Wu   +5 more
wiley   +1 more source

The HLA-E Gene encodes two differentially regulated Transcripts and a Cell Surface Protein [PDF]

, 1992
An HLA-E-specific oligonucleotide probe was used to study the expressioonf HLA-E. This probed etects two HLA-E transcripts, 1.8 and 2.7 kb in size, which are present in varying ratios in allt issues and cell lines investigated.
Honka, Thomas, Johnson, Judith P., Person, Susanne, Ulbrecht, Matthias, Weiss, Elisabeth H.   +4 more
core   +1 more source

Harnessing the Therapeutic Potential of Cell Secretomes and Extracellular Vesicles for Craniofacial Regenerative Applications

Journal of Periodontal Research, EarlyView.
The scoping review summarizes the current preclinical and clinical evidence for the use of “cell‐free” therapies in craniofacial (periodontal, bone and soft‐tissue) regeneration. It also aims to highlight key challenges and strategies towards the clinical translation of these therapies.
Siddharth Shanbhag, Magdalena Mayol, Danijel Domic, Madhusudhan Reddy Bobbili, Johannes Grillari, Mariano Sanz, Reinhard Gruber   +6 more
wiley   +1 more source

Joint single-cell DNA accessibility and protein epitope profiling reveals environmental regulation of epigenomic heterogeneity. [PDF]

, 2018
Here we introduce Protein-indexed Assay of Transposase Accessible Chromatin with sequencing (Pi-ATAC) that combines single-cell chromatin and proteomic profiling. In conjunction with DNA transposition, the levels of multiple cell surface or intracellular
Chang, Howard Y, Chen, Xingqi, Choudhry, Hani, Giaccia, Amato J, Greenleaf, William J, LaGory, Edward L, Litzenburger, Ulrike M, Schep, Alicia N, Wei, Yuning   +8 more
core   +2 more sources

WT1 regulates cyclin�A1 expression in K562�cells

Oncology Reports, 2019
The restricted expression of Wilms tumor 1 (WT1) and cyclin A1 (CCNA1) in normal tissues, as opposed to their abnormal expression in leukemia demonstrates the applicability of WT1 and CCNA1 as cancer antigens for immunotherapy, and as markers for prognosis and relapse.
Sony, Pandey, Mustafa, Moazam, Nirmala, Ghimirey, Steven J, Kuerbitz, Gail C, Fraizer   +4 more
openaire   +3 more sources

Functional investigation of the RHD gene promoter: Molecular changes are rarely responsible for variant D phenotype in Thai donors

Transfusion, EarlyView.
Abstract Background While modern technology is increasingly available to identify efficiently the molecular alterations responsible for variant phenotype, a subset of samples still escapes those analyses. In addition to the promoter region, two intronic regions containing GATA1 binding sites within the RHD gene locus were recently shown to modulate the
Caroline Bénech, Pornlada Nuchnoi, Yann Fichou   +2 more
wiley   +1 more source