Results 111 to 120 of about 8,337,710 (302)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Big Ideas Exchange Highlights Struggle for Cyber Advantage [video]

open access: yes, 2019
Naval Postgraduate School (NPS) faculty and students researching the cutting-edge of how the U.S. can gain an advantage in cyberspace shared their game-changing research during the latest Big Ideas Exchange (BIX), Nov.

core   +1 more source

Microscopic analysis of the destruction of passive film on stainless steel caused by sulfide in simulated cooling water

open access: yesCorrosion Reviews
Sulfide often appears in circulating cooling water due to the presence of sulfate reducing bacteria and could affect corrosion behavior of cooling pipe metals such as stainless steel.
Huang Jinrong   +4 more
doaj   +1 more source

Small RNA pathways in mammalian oocytes

open access: yesFEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

open access: yesFEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Entity Authentication and Authenticated Key

open access: yes, 2006
This paper provides the first analytical and practical treatment of entity authentication and authenticated key exchange in the framework of Tree Parity Machines (TPMs).
Exchange With Tree, Markus Volkmer
core  

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

open access: yesFEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Analysis of the Telegram Key Exchange

open access: yes
We describe, formally model, and prove the security of Telegram's key exchange protocols for client-server communications. To achieve this, we develop a suitable multi-stage key exchange security model along with pseudocode descriptions of the Telegram protocols that are based on analysis of Telegram's specifications and client source code.
Martin R. Albrecht   +4 more
openaire   +3 more sources

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