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Primary Kidney Proximal Tubule Cells
2004Primary rabbit kidney epithelial cell cultures can be obtained that express renal proximal tubule functions. Toward these ends, renal proximal tubules are purified from the rabbit kidney by the method of Brendel and Meezan. To summarize, each kidney is perfused with iron oxide, which becomes associated with glomeruli.
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Accumulation of triglycerides in the proximal tubule of the kidney in diabetic coma
Pathology, 2003The present study was initiated by a very recent histochemical observation of lipid accumulation in the renal cortex of a woman who died in a diabetic coma. Two older reports of lipid accumulation in the kidneys of patients who died, most likely in a state of non-regulated diabetes, supported this observation.
Nielsen, H. +3 more
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The ultrastructure of the developing proximal tubule in the rat kidney
Journal of Ultrastructure Research, 1975The ultrastructure of the proximal tubule cells was studied during development of the nephrons in the newborn and adult rat kidney. Cells in nonfiltering nephrons showed few cellular organelles and a large nucleus. In filtering nephrons the surface area of apical, lateral and basal cell membranes was increased in comparison to nonfiltering nephrons ...
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Regulation of gluconeogenesis in swine kidney proximal tubule cells
Molecular and Cellular Biochemistry, 1989Proximal tubule cells were isolated from swine kidney and cultured for periods of more than 30 days. The cells formed confluent monolayers after plating on a collagen surface and they were passaged more than 5 times on this matrix. The cells maintain several metabolic functions of proximal tubule cells, including gluconeogenesis and the ability to ...
S, Ashkar, J, Kennedy, J, Mendicino
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Pressure and flow in proximal tubules of rat kidneys
Experientia, 1962An proximalen Harnkanalchen wurde die Stromungsgeschwindigkeit der Flussigkeit bestimmt. Es wurde die Durchgangszeit eines Oltropfens durch einzelne Kanalchen gemessen. Der Druckabfall im Kanalchen wurde aus Durchgangszeit und Dimension des Kanalchens berechnet.
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Contraluminal bicarbonate transport in the proximal tubule of the rat kidney
Pflügers Archiv - European Journal of Physiology, 1987In order to measure the contraluminal bicarbonate flux in situ we applied the stopped flow capillary microperfusion technique and measured the influx of 14C-bicarbonate buffer into cortical tubular cells at pH 8. It was found that the influx in percent of the starting concentration is larger at 20 mmol/l bicarbonate than at 1 mmol/l, indicating a ...
Ullrich, K., Papavassiliou, F.
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Cell and luminal pH in the proximal tubule of Necturus kidney
American Journal of Physiology-Renal Physiology, 1984Double-barreled, selective microelectrodes filled with liquid ion exchanger were used to determine proximal tubule cell pH (pHcell), luminal pH (pHlum), and peritubular capillary blood pH (pHbl.pt) in Necturus kidney in vivo. The average pHbl.pt of 16 animals was 7.64 +/- 0.3; pHcell was 7.36 +/- 0.02 (n = 50), and pHlum was 7.50 +/- 0.05 (n = 16 ...
G, Planelles +2 more
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Fluid Absorption in the Kidney Proximal Tubule
Physiology, 1987Fluid absorption in the proximal tubule can be driven by a small osmotic difference between the luminal and interstitial fluids because this leaky epithelium has a high water permeability. The osmotic difference is produced by solute absorption, which tends to dilute the luminal fluid and concentrate the interstitial fluid.
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Urate reabsorption in proximal convoluted tubule of the rat kidney
American Journal of Physiology-Legacy Content, 1976Urate reabsorption was examined in the rat utilizing clearance and in vivo microperfusion techniques. In control rats, the fractional excretion of urate averaged 12.5 +/- 1.3% of the filtered load, with 60-70% of perfused urate reabsorbed at 2 mm of perfused tubule length (urate efflux). Increasing the perfusion concentration of urate to 6, 12, and 25
E J, Weinman +3 more
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Energy Compartmentation and Active Transport in Proximal Kidney Tubules
1986The primary work of the kidney is active transport.1 It is a long-standing observation that a linear relationship exists between the rate of sodium reabsorption by the whole kidney and its rate of oxygen consumption.2,3 Since the oxygen is consumed at the mitochondria and the energy for active transport is used by the Na,K-ATPase located at the plasma ...
L J, Mandel, S P, Soltoff, P C, Brazy
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