miR-127 protects proximal tubule cells against ischemia/reperfusion: identification of kinesin family member 3B as miR-127 target. [PDF]
PLoS ONE, 2012 Ischemia/reperfusion (I/R) is at the basis of renal transplantation and acute kidney injury. Molecular mechanisms underlying proximal tubule response to I/R will allow the identification of new therapeutic targets for both clinical settings.
Elia Aguado-Fraile +9 more
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Mitochondrial organization in the developing proximal tubule is controlled by LRRK2 [PDF]
Nature CommunicationsThe proximal tubule of the nephron performs energy-demanding functions such as resorption of water, amino acids and glucose. Formation of the energy-producing machinery is an essential step in proximal tubule epithelial cell differentiation, and this ...
Mohsina Khan +8 more
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Comparative Analysis of Proximal Tubule Cell Sources for In Vitro Studies of Renal Proximal Tubule Toxicity [PDF]
BiomedicinesBackground/Objectives: The kidneys are essential for eliminating drugs and chemicals from the human body and renal epithelial cells are particularly vulnerable to damage caused by xenobiotics and their metabolites. Drug-induced kidney toxicity is a major
Courtney Sakolish +10 more
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Sustained alterations in proximal tubule gene expression in primary culture associate with HNF4A loss [PDF]
Scientific ReportsPrimary cultures of proximal tubule cells are widely used to model the behavior of kidney epithelial cells in vitro. However, de-differentiation of primary cells upon culture has been observed and appreciated for decades, yet the mechanisms driving this ...
Asha C. Telang +4 more
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Proximal tubule cell maturation rate and function are controlled by PPARα signaling in kidney organoids [PDF]
Communications BiologyHuman pluripotent stem cell-derived kidney organoids are expected to be a useful tool for new drug discoveries, however, the immaturation of kidney organoids causes difficulties in recapitulating renal pharmacokinetics using organoids. Here, we performed
Yoshiki Sahara +3 more
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Stem cells derived from neonatal mouse kidney generate functional proximal tubule-like cells and integrate into developing nephrons in vitro. [PDF]
PLoS ONE, 2013 We have recently shown that kidney-derived stem cells (KSCs) isolated from the mouse newborn kidney differentiate into a range of kidney-specific cell types.
Egon Ranghini +5 more
doaj +4 more sources
Generation of proximal tubule spheroids for nephrotoxicity assessment [PDF]
Organoid, 2022 Background To date, nephrotoxicity in new drug development has been evaluated through two-dimensional culture of representative cell lines, such as HK-2 and human proximal tubule epithelial cells (hPTECs). Approximately 20% of new drugs that were safe in
Dae Hun Kim +3 more
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Prevalence of nephropathy and proximal tubule disorder in human immunodeficiency virus patients under tenofovir therapy [PDF]
Journal of Nephropharmacology, 2022 Introduction: Tenofovir is a common therapy in human immunodeficiency virus (HIV) patients. Objective: This study was carried out to determine the prevalence of nephropathy and proximal tubule disorder in HIV patients under tenofovir therapy.
Foroogh Sabzghabaei +5 more
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PLoS ONE, 2022 Metallothionein 3 (MT-3) is a small, cysteine-rich protein that binds to essential metals required for homeostasis, as well as to heavy metals that have the potential to exert toxic effects on cells.
Gazal Kalyan +8 more
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Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse
Frontiers in Cell and Developmental Biology, 2023 Protein reabsorption in renal proximal tubules is essential for maintaining nutrient homeostasis. Renal proximal tubule-specific gene knockout is a powerful method to assess the function of genes involved in renal proximal tubule protein reabsorption ...
Shiting Liang +7 more
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