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Efficient kidney gene transfer and proximal tubule transduction using self-complementary AAV.cc47 vectors. [PDF]

open access: yesMol Ther Methods Clin Dev
Peek JL   +8 more
europepmc   +1 more source

Construction, evaluation, and applications of renal barrier-on-a-chip system. [PDF]

open access: yesBioact Mater
Naren T   +10 more
europepmc   +1 more source
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Significant water absorption goes paracellular in kidney proximal tubules

American Journal of Physiology-Renal Physiology, 2014
it had been a fundamental dispute for several decades, whether water passes epithelia such as kidney proximal tubule or small intestine along the transcellular, paracellular, or both pathways.
Rita, Rosenthal, Michael, Fromm
openaire   +2 more sources

Regulation of gluconeogenesis in swine kidney proximal tubule cells

Molecular and Cellular Biochemistry, 1989
Proximal tubule cells were isolated from swine kidney and cultured for periods of more than 30 days. The cells formed confluent monolayers after plating on a collagen surface and they were passaged more than 5 times on this matrix. The cells maintain several metabolic functions of proximal tubule cells, including gluconeogenesis and the ability to ...
S, Ashkar, J, Kennedy, J, Mendicino
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Electrophysiological heterogeneity of proximal convoluted tubules in Amphiuma kidney

American Journal of Physiology-Renal Physiology, 1986
The present study was designed to identify functional differences between dark (early to mid) and white (late) proximal tubule segments in Amphiuma kidney. The potential difference across the peritubular cell membrane (Vb), the luminal cell membrane (Va), and the epithelium (Vte) are not significantly different between dark and white segments ...
F, Lang, H, Oberleithner, G, Giebisch
openaire   +2 more sources

Secretion of tetraethylammonium by proximal tubules of rabbit kidneys

American Journal of Physiology-Renal Physiology, 1983
The secretory transport of tetraethylammonium (TEA) was investigated in perfused and nonperfused isolated S1, S2, and S3 segments of proximal tubules from rabbit kidneys. In the perfused tubules the transepithelial net secretory flux and in nonperfused tubules the TEA cellular uptake were saturable (Km = 67 microM, Vmax = 2,480 fmol X min-1 X mm-1 in ...
C, Schäli   +3 more
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Free-flow potential profile along rat kidney proximal tubule

Pfl�gers Archiv European Journal of Physiology, 1974
The transepithelial electrical potential difference across rat rena proximal tubule was reinvestigated, using improved techniques. To diminish tip potential artefacts the microelectrodes were filled with HCO3-Ringer's solution instead of 3 molar KCl. The error of the potential measurements with HCO3-Ringer's microelectrodes was tested and was found to ...
Frömter, E., Geßner, K.
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Micropuncture study of proximal tubule pH in avian kidney

American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 1987
Transepithelial potentials (PD) and pH gradients were measured by in vivo micropuncture in superficial proximal tubules of anesthetized European starlings. The average PD for 46 tubules was 2.24 +/- 1.17 mV (mean +/- SD), lumen negative. Only a single lumen-positive potential was recorded, even though late proximal segments are accessible to ...
G, Laverty, M, Alberici
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Protein degradation in kidney proximal tubule cell monolayers

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1994
Isolated proximal tubule cells have been labelled with L-[4,5-3H]leucine prior to cell division. Histochemical staining demonstrated the purity of the cultures. The bicarbonate ion or a collagen support was required for cell growth. Different culture growth rates were established by varying these parameters.
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