Efficient kidney gene transfer and proximal tubule transduction using self-complementary AAV.cc47 vectors. [PDF]
Peek JL +8 more
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Red yeast rice supplement containing silica nanoparticles induces renal injury in rats with unilateral nephrectomy. [PDF]
Abe M +4 more
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Construction, evaluation, and applications of renal barrier-on-a-chip system. [PDF]
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Significant water absorption goes paracellular in kidney proximal tubules
American Journal of Physiology-Renal Physiology, 2014it had been a fundamental dispute for several decades, whether water passes epithelia such as kidney proximal tubule or small intestine along the transcellular, paracellular, or both pathways.
Rita, Rosenthal, Michael, Fromm
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Regulation of gluconeogenesis in swine kidney proximal tubule cells
Molecular and Cellular Biochemistry, 1989Proximal tubule cells were isolated from swine kidney and cultured for periods of more than 30 days. The cells formed confluent monolayers after plating on a collagen surface and they were passaged more than 5 times on this matrix. The cells maintain several metabolic functions of proximal tubule cells, including gluconeogenesis and the ability to ...
S, Ashkar, J, Kennedy, J, Mendicino
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Electrophysiological heterogeneity of proximal convoluted tubules in Amphiuma kidney
American Journal of Physiology-Renal Physiology, 1986The present study was designed to identify functional differences between dark (early to mid) and white (late) proximal tubule segments in Amphiuma kidney. The potential difference across the peritubular cell membrane (Vb), the luminal cell membrane (Va), and the epithelium (Vte) are not significantly different between dark and white segments ...
F, Lang, H, Oberleithner, G, Giebisch
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Secretion of tetraethylammonium by proximal tubules of rabbit kidneys
American Journal of Physiology-Renal Physiology, 1983The secretory transport of tetraethylammonium (TEA) was investigated in perfused and nonperfused isolated S1, S2, and S3 segments of proximal tubules from rabbit kidneys. In the perfused tubules the transepithelial net secretory flux and in nonperfused tubules the TEA cellular uptake were saturable (Km = 67 microM, Vmax = 2,480 fmol X min-1 X mm-1 in ...
C, Schäli +3 more
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Free-flow potential profile along rat kidney proximal tubule
Pfl�gers Archiv European Journal of Physiology, 1974The transepithelial electrical potential difference across rat rena proximal tubule was reinvestigated, using improved techniques. To diminish tip potential artefacts the microelectrodes were filled with HCO3-Ringer's solution instead of 3 molar KCl. The error of the potential measurements with HCO3-Ringer's microelectrodes was tested and was found to ...
Frömter, E., Geßner, K.
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Micropuncture study of proximal tubule pH in avian kidney
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 1987Transepithelial potentials (PD) and pH gradients were measured by in vivo micropuncture in superficial proximal tubules of anesthetized European starlings. The average PD for 46 tubules was 2.24 +/- 1.17 mV (mean +/- SD), lumen negative. Only a single lumen-positive potential was recorded, even though late proximal segments are accessible to ...
G, Laverty, M, Alberici
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Protein degradation in kidney proximal tubule cell monolayers
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1994Isolated proximal tubule cells have been labelled with L-[4,5-3H]leucine prior to cell division. Histochemical staining demonstrated the purity of the cultures. The bicarbonate ion or a collagen support was required for cell growth. Different culture growth rates were established by varying these parameters.
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