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C5aR Expression in Kidney Tubules, Macrophages and Fibrosis
Journal of Histotechnology, 2023The anaphylatoxin C5a and its receptor C5aR (CD88) are complement pathway effectors implicated in renal diseases, including ANCA-associated vasculitis. We investigated the kidney expression of C5aR and a second C5a receptor C5L2 by using immunohistochemistry and in situ hybridization on formalin-fixed, paraffin-embedded human and mouse kidney. C5aR was
Carolyn Dunlap+4 more
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Isolation of viable rat kidney tubules
Life Sciences, 1971Abstract A method has been devised for the isolation of viable tubules from the rat renal cortex. More than 70% of the preparation consisted of fragments of proximal tubules which respired in calcium- and glucose-free buffer solution. The addition of glucose or succinate to the medium enhanced the oxygen uptake and allowed for a linear respiration ...
Helen R. Strausser, Ho Sam Ahn
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Dextran is resistant to lysosomal digestion in kidney tubules [PDF]
Low molecular weight dextran (Rheomacrodex) was infused into dextran resistant rats in a dose of 5 g/kg body weight. The kidneys were studied by electron microscopy at different time intervals after infusion using a special fixative for the demonstration of dextran.
Christensen, Erik Ilsø, Maunsbach, A B
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[12] Microdissection of kidney tubule segments
1990Publisher Summary This chapter focuses on the micro dissection of kidney tubule segments. Microdissection of fresh kidney tissue and isolation of individual tubule segments is a valuable experimental technique to determine the metabolic characteristics of these renal tubule segments.
P A Wright+2 more
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Electrolyte transport in kidney tubule cells
Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1971Transtubular movement of Na and K takes place across an electrically negative cell compartment rich in K and poor in Na. Some properties of the luminal and peritubular cell boundaries with respect to ionic pump and leak characteristics are analysed. Sodium enters the tubule cell from the lumen down an electrochemical potential gradient.
G. Whittembury+2 more
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On the mechanisms of transfilter induction of kidney tubules
Journal of Theoretical Biology, 1979Abstract Induction of tubule formation in metanephrogenic mesenchyme by spinal cord, occurs across 15 μm thick nucleopore filters with pores greater than 0·1 μm in diameter, but not when the pores are less than 0·05 μm. Processes from the inducer cells penetrate the 0·1 μm pores but not those of 0·05 μm diameter. These results are usually interpreted
Leonard Weiss, Shlomo Nir
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Acidification in the distal tubule of the Amphiuma kidney
Acta Physiologica Scandinavica, 1983The acidification of the urine within the distal tubule of Amphiuma means was studied with micropuncture techniques. Antimony microelectrodes calibrated in vivo were used for measuring the intratubular pH and samples were taken for the determination of Na+‐ and Cl‐‐concentrations and for determining buffer lines within the HCO‐3‐CO2 buffer system.
Bo-Eric Persson, A. Erik G. Persson
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Regenerating Tubules for Kidney Repair
2010Stem/progenitor cells are in the focus of regenerative medicine for a future therapy of acute and chronic renal failure. However, broad knowledge about parenchymal regeneration in kidney is lacking. For that reason developmental pathways leading from stem/progenitor cells to newly formed tubules have to be investigated.
Lucia Denk+2 more
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Lipids in the Proximal Tubules of the Kidney in Diabetic Coma
The American Journal of Forensic Medicine and Pathology, 2000Vacuolization of the renal tubular epithelial cells (the Armanni-Ebstein lesion) associated with diabetic hyperglycemia is usually regarded as an accumulation of glycogen. In a case of death of diabetic coma, the vacuoles were stained strongly for lipids.
Thomsen, J.L., Hansen, T.P.
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